Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 3052135..3052777 Replicon chromosome
Accession NZ_CP094624
Organism Bacillus thuringiensis strain LX43

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag MTX16_RS15780 Protein ID WP_000635963.1
Coordinates 3052427..3052777 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag MTX16_RS15775 Protein ID WP_000004570.1
Coordinates 3052135..3052422 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTX16_RS15750 3047452..3048414 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
MTX16_RS15755 3048407..3048979 - 573 WP_061456689.1 rhomboid family intramembrane serine protease -
MTX16_RS15760 3049073..3049432 + 360 WP_000583416.1 holo-ACP synthase -
MTX16_RS15765 3049589..3050539 + 951 WP_002180340.1 outer membrane lipoprotein carrier protein LolA -
MTX16_RS15770 3050657..3051826 + 1170 WP_000390615.1 alanine racemase -
MTX16_RS15775 3052135..3052422 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
MTX16_RS15780 3052427..3052777 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
MTX16_RS15785 3052845..3055013 + 2169 WP_061456688.1 Tex family protein -
MTX16_RS15790 3055071..3055187 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
MTX16_RS15795 3055383..3055841 + 459 WP_000344234.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T239964 WP_000635963.1 NZ_CP094624:3052427-3052777 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T239964 NZ_LS483422:c890452-890249 [Providencia heimbachae]
ATGACCAAATTTGATTATCTAATGCGTTTAAGAAAATGCACAACAATTGAAACACTTGAGCGTGTCATTGAAAAAAATAA
GTACGAGCTCTCTGAAGATGAGCTGGAGTTATTTTACTCAGCTGCTGATCATCGCCTAGCTGAGCTGACAATGAATAAAC
TTTACGACAAGATACCACCATCTGTTTGGAAGTTTGTTAGATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT239964 WP_000004570.1 NZ_CP094624:3052135-3052422 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT239964 NZ_LS483422:c890891-890523 [Providencia heimbachae]
ATGGATGAATATTCACCCAAAAAACATGATATAGCAGAGTTAAAGTATTTATGTAACAGTCTGAATCGAGACGCAATTTT
AAGTTTACAAAAAACGAATACACACTGGATAAATGATCTCAGCTCACAACAAAGTGCCAATCTCAATGAACTTATCGAGC
ATATTGCTGCTTTTGTTTGGCGATTTAAAATTAAATATCCAAAAGAGAATCTCGTTATATCATTAGTTGAAGAGTATCTT
GACGAGACCTATGACTTATTCGGTAGCCCTGTAATAACGCTTAGTGAGATTATTGACTGGGAAAGCATGAACCAAAATCT
TGTCTCTGTTCTGGAAGATGATTTAAAATGTCTGACCAGTAAAACGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References