Detailed information of TA system TA239924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 7567964..7568637
• Replicon: chromosome
• Accession: NZ_CP094516
• Organism: Bradyrhizobium sp. ISRA430

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MTX21_RS35595
• Protein ID: WP_280969128.1
• Coordinates: 7568215..7568637 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MTX21_RS35590
• Protein ID: WP_280971339.1
• Coordinates: 7567964..7568218 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTX21_RS35575 (MTX21_35430)	7564661..7565350	+	690	WP_280970898.1	YopT-type cysteine protease domain-containing protein	-
MTX21_RS35580 (MTX21_35435)	7566846..7567172	+	327	WP_280969126.1	IS66 family insertion sequence element accessory protein TnpB	-
MTX21_RS35585 (MTX21_35440)	7567263..7567892	+	630	Protein_7061	IS66 family transposase zinc-finger binding domain-containing protein	-
MTX21_RS35590 (MTX21_35445)	7567964..7568218	+	255	WP_280971339.1	plasmid stabilization protein	Antitoxin
MTX21_RS35595 (MTX21_35450)	7568215..7568637	+	423	WP_280969128.1	type II toxin-antitoxin system VapC family toxin	Toxin
MTX21_RS35600 (MTX21_35455)	7568913..7569233	-	321	Protein_7064	transposase	-
MTX21_RS35605 (MTX21_35460)	7570124..7571668	-	1545	WP_280969129.1	acyl-CoA dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	7548475..7571740	23265
	-	flank	IS/Tn	-	-	7566846..7567172	326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 14900.11 Da
• Isoelectric Point: 5.7030

>T239924 WP_280969128.1 NZ_CP094516:7568215-7568637 [Bradyrhizobium sp. ISRA430]
MILLDTNVVSEAMKPDPHPTVRDWLDAQAAETLFLSSVTIAELLFGIGALPRGKRKDRLAAALDGVLDLFATRILPFDTD
AARRYAELAVKARTAGKGFPTPDGYIAAIAAAHGFAVASRDTSAFNAVGLTMIDPWTVTS

Nucleotide

• Length: 423 bp

>T239924 NZ_LS483398:c2890292-2890041 [Bordetella pertussis]
ATTGGAGGCGCGGCGACAAAAAAGCCGGGACAAAAGCGTCGCGCCGGGGCGGCGGCGCTGGGGCTGGATTTCAGCGCCAT
GGTCGAAATCGTGCTTGCATTGACACCGCGGGATTTCCACAAAAGCATGACTACCCATGCCGACCATCGCATTTGGCAGG
ACGTCTATTGCCCGGTGACAGCGATGGGCGCCGTCTACCTGAAGCTGACGGTCCTCGACGGCGTGCCGATCGTGTCGTTC
AAGGAGTTGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9274.55 Da
• Isoelectric Point: 7.3225

>AT239924 WP_280971339.1 NZ_CP094516:7567964-7568218 [Bradyrhizobium sp. ISRA430]
MAAVTIRNLSEEAHRALKVRAAQHNRSAEAEMRAILEAAVRPEGRLQLGTALSEMSRKIGLTNADVEALEHGRDARPAEP
MRFE

Nucleotide

• Length: 255 bp

>AT239924 NZ_LS483398:c2890038-2889637 [Bordetella pertussis]
ATGAAGTGTCCCGTATGCGGAGCGGCCGCGCTGGCGCGTGATACTCGCGATGTGCCCTATAGCTACAAGGGCGAGACCAT
CACGATTGCGGCGGTGACGGGCGACTGGTGTCCGGCATGCGGCGAGAACGTGCTCGATGGCGCTGAATCGGCGCGGGTGA
GCGAGGCCATGCTGGCTTTCAACCGCCTGGTCAACGCCCGCCGCGTCGACCCCGGGTTCATCGTGCGGGTGCGCAGGAAG
CTGGGGCTGGACCAGCGCGAAGCCGCGGCGCTGTTCGGCGGCGGCACCAATGCGTTCTCGCGTTACGAGAACGGCAAGAC
GCGCCCGCCGCTGGCGCTGGTGAAGCTGCTCAGGCTGCTGGATCGCCATCCGGAGCTGCTGGAGGAAGTCCGTGCGGATT
GA