Detailed information of TA system TA239923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2294872..2295545
• Replicon: chromosome
• Accession: NZ_CP094515
• Organism: Bradyrhizobium sp. ISRA426

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MTX24_RS10525
• Protein ID: WP_280969128.1
• Coordinates: 2295123..2295545 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MTX24_RS10520
• Protein ID: WP_280969127.1
• Coordinates: 2294872..2295126 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTX24_RS10505 (MTX24_10460)	2291569..2292258	+	690	WP_280970898.1	YopT-type cysteine protease domain-containing protein	-
MTX24_RS10510 (MTX24_10465)	2293754..2294080	+	327	WP_280969126.1	IS66 family insertion sequence element accessory protein TnpB	-
MTX24_RS10515 (MTX24_10470)	2294171..2294800	+	630	Protein_2079	IS66 family transposase zinc-finger binding domain-containing protein	-
MTX24_RS10520 (MTX24_10475)	2294872..2295126	+	255	WP_280969127.1	plasmid stabilization protein	Antitoxin
MTX24_RS10525 (MTX24_10480)	2295123..2295545	+	423	WP_280969128.1	type II toxin-antitoxin system VapC family toxin	Toxin
MTX24_RS10530 (MTX24_10485)	2295821..2296141	-	321	Protein_2082	transposase	-
MTX24_RS10535 (MTX24_10490)	2297032..2298576	-	1545	WP_280969129.1	acyl-CoA dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2275383..2298648	23265
	-	flank	IS/Tn	-	-	2293754..2294080	326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 14900.11 Da
• Isoelectric Point: 5.7030

>T239923 WP_280969128.1 NZ_CP094515:2295123-2295545 [Bradyrhizobium sp. ISRA426]
MILLDTNVVSEAMKPDPHPTVRDWLDAQAAETLFLSSVTIAELLFGIGALPRGKRKDRLAAALDGVLDLFATRILPFDTD
AARRYAELAVKARTAGKGFPTPDGYIAAIAAAHGFAVASRDTSAFNAVGLTMIDPWTVTS

Nucleotide

• Length: 423 bp

>T239923 NZ_LS483396:c309291-309037 [Micrococcus luteus NCTC 2665]
ATGCGCCTCGTGTCGGACCGCTCCGCCTGGGATGACTGCACGCACTGGCAGACCACGGACCGCAAGGTCCTCCAACGGAT
CAACACCCTCATCGACGCCGCCCTGCGTGATCCCCTCGACGGGATCGGCAAACCCGAGCAGCTGAAGTACGGCGCGGCCG
GGGCATGGTCCCGACGCATCACGGAGGAGCACAGGCTCGTCTACCTCGTCGACGGCGAGGACCTGATCATCCTCCAGGCG
AGGTATCACTACTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9265.54 Da
• Isoelectric Point: 7.2070

>AT239923 WP_280969127.1 NZ_CP094515:2294872-2295126 [Bradyrhizobium sp. ISRA426]
MAAVTIRNLSEEAHRALKVRAAQHNRSAEAEMRAILEAAVRPEGRLQLGTALSEMSRKIGLTNADVEALEQGRDARPAEP
MRFE

Nucleotide

• Length: 255 bp

>AT239923 NZ_LS483396:c309495-309292 [Micrococcus luteus NCTC 2665]
GTGCCTCCCGAGTTCGACCTCGCCGACTGGTACATAAGCAGGCACCACATCGCGCCGGAGGAGGCCCCCATGTCCATCAG
TGCCAGTGAAGCCCGCAAGACCCTGTTCCCGCTCATCGAGCGAGTGACTGCGGACCGCGACGCCGTCGAGATCGTCTCCC
GCAAGGGCAACGCCGTTCTCATGCCCGCGGACGGGGACGCCTGA