Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 18030..18673 Replicon plasmid pSHX180-1
Accession NZ_CP094513
Organism Klebsiella pneumoniae strain SHX180

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag MTX44_RS25155 Protein ID WP_001044768.1
Coordinates 18257..18673 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag MTX44_RS25150 Protein ID WP_001261287.1
Coordinates 18030..18260 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTX44_RS25130 (MTX44_25085) 13190..14278 + 1089 WP_000952217.1 transcriptional repressor PifC -
MTX44_RS25135 (MTX44_25090) 14280..16505 + 2226 WP_000698737.1 P-loop NTPase fold protein -
MTX44_RS25140 (MTX44_25095) 16555..17454 - 900 WP_000963206.1 nucleotide-binding protein -
MTX44_RS25145 (MTX44_25100) 17444..17734 - 291 WP_000111771.1 hypothetical protein -
MTX44_RS25150 (MTX44_25105) 18030..18260 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MTX44_RS25155 (MTX44_25110) 18257..18673 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
MTX44_RS25160 (MTX44_25115) 18835..20973 - 2139 WP_000350638.1 AAA family ATPase -
MTX44_RS25165 (MTX44_25120) 21327..21584 + 258 WP_000343085.1 hypothetical protein -
MTX44_RS25170 (MTX44_25125) 21584..22174 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mph(A) / sul1 / qacE / qnrB6 / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / floR / tet(A) / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id - 1..73661 73661


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T239922 WP_001044768.1 NZ_CP094513:18257-18673 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T239922 NZ_LS483395:c5390838-5390557 [Achromobacter xylosoxidans]
GTGCCCGCAGTTGAATGGTCGTCCGCCGCCCGGGCCGACCTGTTGGCCATAGTCGACTACATATCAGATGACAATCCAGA
CGCTGCCCAGCGTCTGAAAGACGATATTGAAACGAAGGCGGCGCAACTGCCCGCCCGTCCCGCGCTGTACCGCCCCGGAA
GGATCGCCGGTACCCGCGAAATGGTCGTTCGCGCGAATTACATCATCGTGTATGCGGCGGATGCGCTGGCCGTGCGGATC
CTGCGCATCTTGCATGCCGCGCAGCAATGGCCGCCGCAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT239922 WP_001261287.1 NZ_CP094513:18030-18260 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT239922 NZ_LS483395:c5391121-5390825 [Achromobacter xylosoxidans]
ATGCCCCAGACCTCGATGCTCCACGTCCGCGTGGACGACAAGCTCAAAGCCGACGCCACCGAAAAGCTGGCCGAATTCGG
CCTGACGATTTCCGACGCCGTGCGCATCCTGCTCACGCGGGTGGCCAAGGAGGGCGGCCTGCCCGCCGGGTTGACCACCG
ATGCCCAGGCGCACGACGCGTGGTTCCGCGCCAAAGTCCAGGAAGCCCTGGCCGATACGGGGCCGGCGGTGCCGCACCAG
CAGGTCATGGATGAAGCCCAGGCGCTGATCGACAGGAAGCGCCGTGCCCGCAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References