Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5107699..5108324 Replicon chromosome
Accession NZ_CP094512
Organism Klebsiella pneumoniae strain SHX180

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MTX44_RS24685 Protein ID WP_002882817.1
Coordinates 5107699..5108082 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MTX44_RS24690 Protein ID WP_004150355.1
Coordinates 5108082..5108324 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTX44_RS24670 (MTX44_24625) 5105065..5105967 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MTX44_RS24675 (MTX44_24630) 5105964..5106599 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MTX44_RS24680 (MTX44_24635) 5106596..5107525 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MTX44_RS24685 (MTX44_24640) 5107699..5108082 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MTX44_RS24690 (MTX44_24645) 5108082..5108324 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MTX44_RS24695 (MTX44_24650) 5108529..5109446 + 918 WP_004187175.1 alpha/beta hydrolase -
MTX44_RS24700 (MTX44_24655) 5109460..5110401 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MTX44_RS24705 (MTX44_24660) 5110446..5110883 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MTX44_RS24710 (MTX44_24665) 5110880..5111740 - 861 WP_004146232.1 virulence factor BrkB family protein -
MTX44_RS24715 (MTX44_24670) 5111734..5112333 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T239921 WP_002882817.1 NZ_CP094512:c5108082-5107699 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T239921 NZ_LS483395:c4334067-4333792 [Achromobacter xylosoxidans]
ATGGAAATGAAGTGGACCAGCAAGGCGCTTTCCGATCTGGCGCGCTTGCATGAATTCCTGGCGGCGGTGAATAGGCCCGC
GGCCGTACGGGCTGTGCAAGCGCTGGTCAAGGCTTCAAGTGTTTTGCCGACCAACTCCCGCATTGGCGAGCAGCTATTTC
AGTTCGAACCGCGCGAGGTACGCAGGATATTGGTCGGGGAGTACGAAATACGTTACGAGATCCGGGATTCGATCATCTAC
GTATTGCGACTGTGGCACACCCGGGAGAACCGCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT239921 WP_004150355.1 NZ_CP094512:c5108324-5108082 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT239921 NZ_LS483395:c4334333-4334067 [Achromobacter xylosoxidans]
ATGAGTCCCGCAACCAAGGTGCTGACCGCACATGTGCCGCTTCCGTTGGCCGAGAAGGTCGATCAGATGGCTGTGCGCCT
GGACCGATCTCGCGGCTGGATCGTGAAGCAGGCACTGTCCGCTTGGATTGCCCAAGAGGAGGAACGTGACAAGCTCACCG
AGCAAGCGCTTGCTGACGTGGATGCTGGTCGGATTGTGGATGACCAAGCCGTGCAAGCCTGGGCGGAGAGCCTGGGCTCT
GACCAGCCATTGCCGCTGCCGCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References