Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4506013..4506823 Replicon chromosome
Accession NZ_CP094512
Organism Klebsiella pneumoniae strain SHX180

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag MTX44_RS21945 Protein ID WP_004178461.1
Coordinates 4506013..4506546 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag MTX44_RS21950 Protein ID WP_002887278.1
Coordinates 4506557..4506823 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTX44_RS21940 (MTX44_21900) 4504844..4505965 + 1122 WP_213222846.1 cupin domain-containing protein -
MTX44_RS21945 (MTX44_21905) 4506013..4506546 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
MTX44_RS21950 (MTX44_21910) 4506557..4506823 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MTX44_RS21955 (MTX44_21915) 4506926..4508359 - 1434 WP_042936879.1 Cu(+)/Ag(+) sensor histidine kinase -
MTX44_RS21960 (MTX44_21920) 4508349..4509032 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
MTX44_RS21965 (MTX44_21925) 4509204..4510589 + 1386 WP_156659814.1 efflux transporter outer membrane subunit -
MTX44_RS21970 (MTX44_21930) 4510607..4510951 + 345 WP_156659816.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T239919 WP_004178461.1 NZ_CP094512:c4506546-4506013 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T239919 NZ_LS483395:1077290-1077667 [Achromobacter xylosoxidans]
ATGATCCTGGTTGACACTTCCATCTGGATAGATCATTTATCCGACGGCGAGCCATGTCTGGTGCGCTTGCTGGAGGAAGA
AAGTGTATTGATGCACCCGTTCATCGCGGGTGAATTGGCGCTGGGTTCGTTGGGCCGGCGTGACGTTGTATTGGGTACGA
TGTCGCTATTGCCTCAAGTCGTGCGTGCGAGTCATGACGAAGTCATGCACTTTCTTCATAGCGAGAGATTGTTTGGAAAG
GGCATTGGTTATGTGGATTTACACTTGTTGGCGTCCACGCGACTGACACCTGGCGCGGCGCTATGGACCAGGGACAAACG
GCTGCAGGCACTCGCCACCACCTTGAGCCTGGCGGTTGATCCCCCGAGATATCATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT239919 WP_002887278.1 NZ_CP094512:c4506823-4506557 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT239919 NZ_LS483395:1077094-1077303 [Achromobacter xylosoxidans]
GTGCGTACGACTGTGACGCTCGATGACGACCTGCTCGAATTGGCCCGTACCTATACGGGCGTCCAGGAGACATCGGCGCT
CGTAACGCGAGCGCTGACCCAGCTCGTGCAGAAAGAGGCCGCGAAGCGGCTGGCCAAGCTTGGTGGTAGTTCGCCCAAGT
TGAAGCCTATCCCGCGGCGGCAGACGGAAACCTGCAATGATCCTGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References