Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4606295..4607105 Replicon chromosome
Accession NZ_CP094479
Organism Klebsiella pneumoniae strain LXR-1

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag MST28_RS22650 Protein ID WP_004178461.1
Coordinates 4606295..4606828 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag MST28_RS22655 Protein ID WP_002887278.1
Coordinates 4606839..4607105 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MST28_RS22645 (4605126) 4605126..4606247 + 1122 WP_002887282.1 cupin domain-containing protein -
MST28_RS22650 (4606295) 4606295..4606828 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
MST28_RS22655 (4606839) 4606839..4607105 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MST28_RS22660 (4607208) 4607208..4608641 - 1434 WP_021463709.1 Cu(+)/Ag(+) sensor histidine kinase -
MST28_RS22665 (4608631) 4608631..4609314 - 684 WP_020804437.1 copper response regulator transcription factor CusR -
MST28_RS22670 (4609486) 4609486..4610871 + 1386 WP_221013835.1 efflux transporter outer membrane subunit -
MST28_RS22675 (4610889) 4610889..4611221 + 333 WP_025368487.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T239897 WP_004178461.1 NZ_CP094479:c4606828-4606295 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T239897 NZ_LS483384:1585589-1585924 [Streptococcus pyogenes]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCTCA
AAACTACTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAGCGACATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCAGCCATTATCACAGCACTAAAGGTTACACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGGGGAAGAAAATAGGGTCGTTATTGATTACTTGCTTCCTACTCAAAGCGACTATAT
AAAGTTATTCAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT239897 WP_002887278.1 NZ_CP094479:c4607105-4606839 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT239897 NZ_LS483384:1585312-1585599 [Streptococcus pyogenes]
ATGACTACAGTAAAAAAAAACAGAGCTGTTACCTTTCAAGCTAATAAAGAATTGGTAAGTGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAACAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References