Detailed information of TA system TA023987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4655757..4656358
• Replicon: chromosome
• Accession: NC_012917
• Organism: Pectobacterium carotovorum subsp. carotovorum PC1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: C6DIE6
• Locus tag: PC1_RS20610
• Protein ID: WP_015842212.1
• Coordinates: 4655975..4656358 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: C6DIE5
• Locus tag: PC1_RS20605
• Protein ID: WP_010300679.1
• Coordinates: 4655757..4655978 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PC1_RS20570	4651985..4652629	+	645	WP_015842205.1	hypothetical protein	-
PC1_RS20575	4652735..4652989	+	255	WP_015842206.1	hypothetical protein	-
PC1_RS20580	4653037..4653615	+	579	WP_015842207.1	hypothetical protein	-
PC1_RS20585	4653773..4654090	+	318	WP_015842208.1	helix-turn-helix domain-containing protein	-
PC1_RS20590	4654106..4654363	-	258	WP_015842209.1	helix-turn-helix domain-containing protein	-
PC1_RS20595	4654461..4654796	+	336	WP_015842210.1	helix-turn-helix domain-containing protein	-
PC1_RS20600	4654936..4655481	-	546	WP_015842211.1	HNH endonuclease	-
PC1_RS20605	4655757..4655978	+	222	WP_010300679.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PC1_RS20610	4655975..4656358	+	384	WP_015842212.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
PC1_RS20615	4656521..4657078	+	558	WP_015842213.1	hypothetical protein	-
PC1_RS20620	4657126..4657677	-	552	WP_043881846.1	hypothetical protein	-
PC1_RS20625	4657785..4658171	+	387	WP_015842215.1	hypothetical protein	-
PC1_RS22105	4658210..4658293	+	84	Protein_4080	DUF596 domain-containing protein	-
PC1_RS20630	4658304..4659503	-	1200	WP_043881847.1	AI-2E family transporter	-
PC1_RS20635	4659597..4660097	-	501	WP_015842217.1	GrpB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14347.29 Da
• Isoelectric Point: 7.3172

>T23987 WP_015842212.1 NC_012917:4655975-4656358 [Pectobacterium carotovorum subsp. carotovorum PC1]
MNWVSAQEVIAFHDRILRRLPGVAGMPDPGRAEAIIYRVQNRTHYEGITDIFELAATYWVAVARGHIFNDGNKRTAFFVT
MTFLYRNGVLVRDNDNTLENLTVEAATGAKNVDELAQQLRTRVQPLG

Nucleotide

• Length: 384 bp

>T23987 NC_012917:4655975-4656358 [Pectobacterium carotovorum subsp. carotovorum PC1]
ATGAACTGGGTTAGCGCACAAGAAGTGATCGCCTTTCATGATCGGATATTGCGACGTTTACCCGGCGTAGCGGGAATGCC
CGATCCGGGGCGAGCAGAAGCGATAATCTACCGAGTGCAGAACCGCACGCATTATGAAGGGATCACGGACATCTTCGAGC
TGGCCGCCACTTACTGGGTGGCTGTTGCACGTGGCCATATTTTTAATGATGGTAATAAGCGAACAGCATTCTTCGTCACG
ATGACGTTTTTATACCGTAACGGCGTGCTGGTTCGCGATAACGACAATACGTTGGAAAATCTGACGGTAGAGGCCGCAAC
GGGTGCAAAAAACGTTGACGAACTGGCACAGCAATTGAGAACACGGGTGCAACCTTTAGGCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8135.01 Da
• Isoelectric Point: 5.0359

>AT23987 WP_010300679.1 NC_012917:4655757-4655978 [Pectobacterium carotovorum subsp. carotovorum PC1]
MRTYTSTQARANISEVLDAATHGEPVEITRRDGSSAVVISKAEFEAYQNAKLDAEFDLIMQRHGHTIEALTDR

Nucleotide

• Length: 222 bp

>AT23987 NC_012917:4655757-4655978 [Pectobacterium carotovorum subsp. carotovorum PC1]
ATGCGAACCTACACTTCTACTCAAGCCAGAGCCAACATTTCAGAAGTATTAGATGCAGCAACCCACGGTGAACCTGTCGA
AATTACTCGTCGGGATGGAAGCTCTGCGGTGGTGATCAGCAAAGCGGAATTCGAGGCATACCAAAATGCCAAGTTAGACG
CCGAATTTGATCTCATCATGCAGCGCCACGGCCACACTATCGAAGCGCTGACTGACCGATGA

Structures

Toxin

• AlphaFold DB: C6DIE6

Antitoxin

• AlphaFold DB: A0A221TFG2