Detailed information of TA system TA239868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 1355089..1356307
• Replicon: chromosome
• Accession: NZ_CP094460
• Organism: Burkholderia pyrrocinia strain JK-SH007

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MRS60_RS22975
• Protein ID: WP_243566754.1
• Coordinates: 1355726..1356307 (+)
• Length: 194 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MRS60_RS22970
• Protein ID: WP_243566753.1
• Coordinates: 1355089..1355646 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRS60_RS22945 (MRS60_22945)	1350124..1350714	+	591	WP_243566750.1	isochorismatase family cysteine hydrolase	-
MRS60_RS22950 (MRS60_22950)	1350958..1351689	-	732	WP_034180616.1	ribonuclease T2	-
MRS60_RS22955 (MRS60_22955)	1351930..1352508	-	579	WP_243566751.1	hypothetical protein	-
MRS60_RS22960 (MRS60_22960)	1352603..1354006	-	1404	WP_034180618.1	membrane protein	-
MRS60_RS22965 (MRS60_22965)	1354369..1355007	+	639	WP_243566752.1	hypothetical protein	-
MRS60_RS22970 (MRS60_22970)	1355089..1355646	+	558	WP_243566753.1	helix-turn-helix transcriptional regulator	Antitoxin
MRS60_RS22975 (MRS60_22975)	1355726..1356307	+	582	WP_243566754.1	HD domain-containing protein	Toxin
MRS60_RS22980 (MRS60_22980)	1356594..1357787	+	1194	WP_243566755.1	efflux RND transporter periplasmic adaptor subunit	-
MRS60_RS22985 (MRS60_22985)	1357790..1359820	+	2031	WP_243566756.1	MacB family efflux pump subunit	-
MRS60_RS22990 (MRS60_22990)	1359833..1361206	+	1374	WP_243566757.1	efflux transporter outer membrane subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (6-147)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-44)

Sequences

Toxin

Protein

• Length: 194 a.a.
• Molecular weight: 21310.91 Da
• Isoelectric Point: 5.0938

>T239868 WP_243566754.1 NZ_CP094460:1355726-1356307 [Burkholderia pyrrocinia]
MNPDTIHARLDFLREAERLKDVLRSGHTSAGRAESTAEHSWRLCLMALVFADALPGIDTLKLLKLCVVHDLGEALHGDIP
AIVQAAHPDKSAHERDDLLTLTASLDRTLRDEIVTLWDEYEAAASPEARAAKALDKLETILQHNQGSNPPDFDYAFNLGY
GRRYTDATPLFSAIRDIVDADTQRRIDAGGSRA

Nucleotide

• Length: 582 bp

>T239868 NZ_LS483365:c2511228-2511121 [Staphylococcus aureus subsp. aureus NCTC 8325]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20239.18 Da
• Isoelectric Point: 10.7033

>AT239868 WP_243566753.1 NZ_CP094460:1355089-1355646 [Burkholderia pyrrocinia]
MAASPLHTPPLGPQLKRWRALHRVKQSHAAELFGVAQSTISRWEACIQQMSPDERATAERLLAARLDSAGDHALARLIAG
SAGRMHLVCDLTHRLLASSPARAAEFSQPLSMLLGTSLWRYATPEIVRMEAALDTLGWHDRAGPPSVEFDTGANASRVVP
IRGSRCRWTRMTLSDGSAARLVETR

Nucleotide

• Length: 558 bp

>AT239868 NZ_LS483365:2511044-2511104 [Staphylococcus aureus subsp. aureus NCTC 8325]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA