Detailed information of TA system TA239865

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1073313..1075060
• Replicon: chromosome
• Accession: NZ_CP094460
• Organism: Burkholderia pyrrocinia strain JK-SH007

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: MRS60_RS21580
• Protein ID: WP_175747500.1
• Coordinates: 1074164..1075060 (-)
• Length: 299 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: MRS60_RS21575
• Protein ID: WP_131949451.1
• Coordinates: 1073313..1074167 (-)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRS60_RS21555 (MRS60_21555)	1068471..1069961	-	1491	WP_131949454.1	aldehyde dehydrogenase	-
MRS60_RS21560 (MRS60_21560)	1070051..1070866	-	816	WP_034180364.1	aspartate dehydrogenase	-
MRS60_RS21565 (MRS60_21565)	1070859..1072526	-	1668	WP_131949453.1	thiamine pyrophosphate-binding protein	-
MRS60_RS21570 (MRS60_21570)	1072519..1073316	-	798	WP_243566641.1	SDR family oxidoreductase	-
MRS60_RS21575 (MRS60_21575)	1073313..1074167	-	855	WP_131949451.1	IclR family transcriptional regulator	Antitoxin
MRS60_RS21580 (MRS60_21580)	1074164..1075060	-	897	WP_175747500.1	alpha/beta fold hydrolase	Toxin
MRS60_RS21585 (MRS60_21585)	1075079..1075606	-	528	WP_034180369.1	cupin domain-containing protein	-
MRS60_RS21590 (MRS60_21590)	1075636..1076406	-	771	WP_217587734.1	SDR family oxidoreductase	-
MRS60_RS21595 (MRS60_21595)	1076399..1077469	-	1071	WP_034180371.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
MRS60_RS21600 (MRS60_21600)	1077525..1077836	-	312	WP_034180372.1	hypothetical protein	-
MRS60_RS21605 (MRS60_21605)	1077838..1078155	-	318	WP_027789384.1	non-heme iron oxygenase ferredoxin subunit	-
MRS60_RS21610 (MRS60_21610)	1078603..1079682	+	1080	WP_131949447.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (51-279)

Antitoxin

• Transcription regulator IclR, C-terminal (142-265)

Sequences

Toxin

Protein

• Length: 299 a.a.
• Molecular weight: 30620.85 Da
• Isoelectric Point: 7.7326

>T239865 WP_175747500.1 NZ_CP094460:c1075060-1074164 [Burkholderia pyrrocinia]
MSVIDKRERDRTAAFAALLGQFPEQRCAAGAAGTIGYREAGAQHAGRALPVVLLHGIGSGAASWVRQLDTLGASRRVLAW
DAPGYGASTPVHGASPAAADYAASLNAWLEALGIERCVLVGHSLGAIVAGGLARVMPARIAGLLLVSPAGGYGSAPAETR
ESRRDARLAMLAELGPAGLAEQRSGNMLSGFASEDARAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRTAVAVG
ANDAITPPAACERIAAAAHVGLQVIPQAGHAGYVEAPAVYSALIDTFCRQCDTQWGLV

Nucleotide

• Length: 897 bp

>T239865 NZ_LS483365:c2163529-2163320 [Staphylococcus aureus subsp. aureus NCTC 8325]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31334.86 Da
• Isoelectric Point: 6.5086

>AT239865 WP_131949451.1 NZ_CP094460:c1074167-1073313 [Burkholderia pyrrocinia]
MSEPLQQEPENGKAEASYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYKL
GIAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTTHIVIRDGRDVVFVAKAQSQSPVFSSIRVNVGTRLPAYATTHGHV
LMGDLSLKELRSLYPEGTLNRMTSATPETVDALYEVIREDALRGYGVSNSSFERGISVVTAPVRNETQKIVACITVTVPR
PEIDAALIADGLIDKVQRAAAELSRRLNYRSDDEHTFMKALGLR

Nucleotide

• Length: 855 bp

>AT239865 NZ_LS483365:c2163834-2163541 [Staphylococcus aureus subsp. aureus NCTC 8325]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA