Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 335243..335820 Replicon chromosome
Accession NZ_CP094460
Organism Burkholderia pyrrocinia strain JK-SH007

Toxin (Protein)


Gene name vapC Uniprot ID A0A2S8IKV0
Locus tag MRS60_RS18395 Protein ID WP_034179822.1
Coordinates 335243..335608 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A2S8IKU8
Locus tag MRS60_RS18400 Protein ID WP_034179821.1
Coordinates 335602..335820 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRS60_RS18370 (MRS60_18370) 330914..331468 + 555 WP_175748564.1 carboxymuconolactone decarboxylase family protein -
MRS60_RS18375 (MRS60_18375) 331632..332444 + 813 WP_243566354.1 extensin -
MRS60_RS18380 (MRS60_18380) 332542..334140 - 1599 WP_175748562.1 alkyl hydroperoxide reductase subunit F -
MRS60_RS18385 (MRS60_18385) 334260..334823 - 564 WP_034179823.1 alkyl hydroperoxide reductase subunit C -
MRS60_RS18390 (MRS60_18390) 334889..335212 + 324 WP_235212450.1 GNAT family N-acetyltransferase -
MRS60_RS18395 (MRS60_18395) 335243..335608 - 366 WP_034179822.1 type II toxin-antitoxin system VapC family toxin Toxin
MRS60_RS18400 (MRS60_18400) 335602..335820 - 219 WP_034179821.1 hypothetical protein Antitoxin
MRS60_RS18405 (MRS60_18405) 335947..336843 - 897 WP_034179820.1 LysR substrate-binding domain-containing protein -
MRS60_RS18410 (MRS60_18410) 337021..338187 + 1167 WP_034179819.1 branched-chain amino acid ABC transporter substrate-binding protein -
MRS60_RS18415 (MRS60_18415) 338207..338731 + 525 WP_175748561.1 L-2-amino-thiazoline-4-carboxylic acid hydrolase -
MRS60_RS18420 (MRS60_18420) 338724..339974 + 1251 WP_243566355.1 Zn-dependent hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13537.61 Da        Isoelectric Point: 9.5275

>T239862 WP_034179822.1 NZ_CP094460:c335608-335243 [Burkholderia pyrrocinia]
MVKALFDTNILIDYLGGVESARAELGRYDYRAISTISWMEVLVGTSARNEAPTRAWLSSFDVIALDSTVANRAVIIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPPTEPGVRMPYQI

Download         Length: 366 bp

>T239862 NZ_LS483365:c1899432-1899280 [Staphylococcus aureus subsp. aureus NCTC 8325]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8313.46 Da        Isoelectric Point: 4.4419

>AT239862 WP_034179821.1 NZ_CP094460:c335820-335602 [Burkholderia pyrrocinia]
MSRILVDLSDGQLDELAVIVETERRPRAAIIRDAIDAYIALHKRPLADDVFGLWKDRTVDGLAYQEEMRSEW

Download         Length: 219 bp

>AT239862 NZ_LS483365:c1900056-1899457 [Staphylococcus aureus subsp. aureus NCTC 8325]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S8IKV0

Antitoxin

Source ID Structure
AlphaFold DB A0A2S8IKU8

References