Detailed information of TA system TA023986

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3266478..3267203
• Replicon: chromosome
• Accession: NC_012917
• Organism: Pectobacterium carotovorum subsp. carotovorum PC1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: C6DAT2
• Locus tag: PC1_RS14330
• Protein ID: WP_015841075.1
• Coordinates: 3266883..3267203 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: C6DAT1
• Locus tag: PC1_RS14325
• Protein ID: WP_015841074.1
• Coordinates: 3266478..3266813 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PC1_RS14290	3261666..3262541	+	876	WP_015841068.1	GTPase family protein	-
PC1_RS14295	3262626..3263447	+	822	WP_015841069.1	DUF945 domain-containing protein	-
PC1_RS14300	3263672..3264370	+	699	WP_015841070.1	DeoR family transcriptional regulator	-
PC1_RS14305	3264552..3265121	+	570	WP_015841071.1	hypothetical protein	-
PC1_RS14310	3265157..3265630	+	474	WP_015841072.1	hypothetical protein	-
PC1_RS14315	3265647..3265880	+	234	WP_014916378.1	membrane protein	-
PC1_RS14320	3265965..3266438	+	474	WP_015841073.1	DNA repair protein RadC	-
PC1_RS14325	3266478..3266813	+	336	WP_015841074.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
PC1_RS14330	3266883..3267203	+	321	WP_015841075.1	TA system toxin CbtA family protein	Toxin
PC1_RS14335	3267319..3268152	+	834	WP_015841076.1	DUF4942 domain-containing protein	-
PC1_RS22395	3268334..3268504	+	171	WP_155116481.1	hypothetical protein	-
PC1_RS14340	3268756..3269867	+	1112	Protein_2851	tyrosine-type recombinase/integrase	-
PC1_RS22400	3269884..3270601	-	718	Protein_2852	IS3 family transposase	-
PC1_RS22405	3270602..3270801	-	200	Protein_2853	IS630 family transposase	-
PC1_RS21520	3270940..3271836	-	897	WP_015841077.1	HPr kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3250738..3289146	38408

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12104.79 Da
• Isoelectric Point: 5.0492

>T23986 WP_015841075.1 NC_012917:3266883-3267203 [Pectobacterium carotovorum subsp. carotovorum PC1]
MQTTPAIQSREDQSCPSPVTVWQQLLTYLLEKHYGLTLNDTPFCEENVIQAHIDAGITLVNAVNFLVEKYELVRIDRNGF
NWQEQSPFLTTVDILRARRATGLLKA

Nucleotide

• Length: 321 bp

>T23986 NC_012917:3266883-3267203 [Pectobacterium carotovorum subsp. carotovorum PC1]
ATGCAAACAACACCTGCAATCCAGTCACGGGAGGATCAGTCTTGCCCGTCACCTGTAACCGTCTGGCAGCAACTGCTGAC
CTATCTACTGGAAAAGCACTACGGCCTGACACTGAACGACACACCGTTCTGTGAAGAAAACGTGATTCAGGCGCATATCG
ATGCTGGTATCACGCTGGTCAATGCCGTCAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATTGATCGCAACGGCTTT
AACTGGCAGGAGCAATCACCGTTTCTCACGACTGTCGATATCCTTCGCGCCAGACGTGCAACGGGCTTACTTAAGGCATA
A

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12412.15 Da
• Isoelectric Point: 6.6855

>AT23986 WP_015841074.1 NC_012917:3266478-3266813 [Pectobacterium carotovorum subsp. carotovorum PC1]
MPLSDSPEWGLKRAVTPQFGARLVQEGHYLHYLSDRASIVGTFSKTEAQSLECCFPELIKLLEQKLRTGELNPRQQGCIT
LHCNKWTCEADTLGSFGYVYIAIYPSSAATQ

Nucleotide

• Length: 336 bp

>AT23986 NC_012917:3266478-3266813 [Pectobacterium carotovorum subsp. carotovorum PC1]
ATGCCATTGTCTGATTCCCCGGAATGGGGACTAAAACGTGCCGTTACTCCACAGTTCGGAGCCAGGCTGGTTCAGGAAGG
TCACTATCTTCATTATCTGTCAGATCGGGCCAGCATTGTCGGTACGTTTAGCAAAACAGAAGCCCAAAGTCTGGAATGTT
GCTTCCCTGAGCTGATTAAGCTGTTGGAGCAGAAGCTACGCACGGGTGAACTGAATCCCCGCCAGCAGGGCTGCATCACG
CTGCATTGCAACAAGTGGACCTGTGAAGCCGATACGCTGGGCAGTTTTGGCTACGTGTATATTGCTATTTATCCCTCTTC
AGCGGCAACACAATAA

Structures

Toxin

• AlphaFold DB: C6DAT2

Antitoxin

• AlphaFold DB: C6DAT1