Detailed information of TA system TA239859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2468928..2469898
• Replicon: chromosome
• Accession: NZ_CP094455
• Organism: Bacillus sp. ZJS3

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: C2MKV2
• Locus tag: MQW34_RS12750
• Protein ID: WP_001997496.1
• Coordinates: 2468928..2469665 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: MQW34_RS12755
• Protein ID: WP_000588712.1
• Coordinates: 2469773..2469898 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQW34_RS12725 (MQW34_12755)	2464435..2465136	+	702	WP_001294994.1	hypothetical protein	-
MQW34_RS12730 (MQW34_12760)	2465258..2466142	+	885	WP_210627572.1	hypothetical protein	-
MQW34_RS12735 (MQW34_12765)	2466463..2467245	-	783	Protein_2428	thiamine pyrophosphate-binding protein	-
MQW34_RS12740 (MQW34_12770)	2467352..2467834	+	483	WP_078418323.1	MarR family winged helix-turn-helix transcriptional regulator	-
MQW34_RS12745 (MQW34_12775)	2468002..2468739	+	738	WP_210627573.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
MQW34_RS12750 (MQW34_12780)	2468928..2469665	+	738	WP_001997496.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
MQW34_RS12755 (MQW34_12785)	2469773..2469898	+	126	WP_000588712.1	hypothetical protein	Antitoxin
MQW34_RS12760 (MQW34_12790)	2469987..2470136	+	150	WP_210627948.1	hypothetical protein	-
MQW34_RS12765 (MQW34_12795)	2470174..2470455	-	282	WP_210627947.1	hypothetical protein	-
MQW34_RS12770 (MQW34_12800)	2470746..2470974	-	229	Protein_2435	hypothetical protein	-
MQW34_RS12775	2471101..2471229	+	129	WP_264752955.1	hypothetical protein	-
MQW34_RS12780 (MQW34_12805)	2471306..2471482	+	177	WP_179955797.1	stage II sporulation protein SB	-
MQW34_RS12785 (MQW34_12810)	2471626..2473095	+	1470	WP_243500628.1	beta-Ala-His dipeptidase	-
MQW34_RS12790 (MQW34_12815)	2473677..2474147	-	471	WP_078418318.1	DUF5065 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28377.08 Da
• Isoelectric Point: 8.2672

>T239859 WP_001997496.1 NZ_CP094455:2468928-2469665 [Bacillus sp. ZJS3]
VISNIRIGLFILAIVFLVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTMIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T239859 NZ_LS483365:c812000-811812 [Staphylococcus aureus subsp. aureus NCTC 8325]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT239859 WP_000588712.1 NZ_CP094455:2469773-2469898 [Bacillus sp. ZJS3]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT239859 NZ_LS483365:c812289-812029 [Staphylococcus aureus subsp. aureus NCTC 8325]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A1T2Q3U8

Antitoxin

• AlphaFold DB: A0A366GQT1