Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5587815..5588440 Replicon chromosome
Accession NZ_CP094451
Organism Klebsiella pneumoniae subsp. rhinoscleromatis strain KP4831

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MRR30_RS27625 Protein ID WP_002882817.1
Coordinates 5587815..5588198 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MRR30_RS27630 Protein ID WP_004150355.1
Coordinates 5588198..5588440 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRR30_RS27610 (MRR30_27610) 5585181..5586083 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MRR30_RS27615 (MRR30_27615) 5586080..5586715 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MRR30_RS27620 (MRR30_27620) 5586712..5587641 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MRR30_RS27625 (MRR30_27625) 5587815..5588198 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MRR30_RS27630 (MRR30_27630) 5588198..5588440 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MRR30_RS27635 (MRR30_27635) 5588645..5589562 + 918 WP_002882812.1 alpha/beta hydrolase -
MRR30_RS27640 (MRR30_27640) 5589576..5590517 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MRR30_RS27645 (MRR30_27645) 5590562..5590999 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MRR30_RS27650 (MRR30_27650) 5590996..5591856 - 861 WP_002882807.1 virulence factor BrkB family protein -
MRR30_RS27655 (MRR30_27655) 5591850..5592449 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T239848 WP_002882817.1 NZ_CP094451:c5588198-5587815 [Klebsiella pneumoniae subsp. rhinoscleromatis]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T239848 NZ_LS483358:1461259-1461510 [Actinobacillus pleuropneumoniae]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACAATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATAGTTCACTTGAAGAAACCGCCTATT
TAATGCGTTCCCCGGCTAACGCTCAAAGATTACTAGACAGCATGGCAGAATTGAAGCAAGGGAAAGGCATTGAAAGAGAG
CTAATCGAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT239848 WP_004150355.1 NZ_CP094451:c5588440-5588198 [Klebsiella pneumoniae subsp. rhinoscleromatis]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT239848 NZ_LS483358:1461507-1461767 [Actinobacillus pleuropneumoniae]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGTCTGATTAAAGATATTCAACGCCAACCATTTGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References