Detailed information of TA system TA239830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 5845323..5845958
• Replicon: chromosome
• Accession: NZ_CP094446
• Organism: Paenibacillus thiaminolyticus strain Mbale2

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: L3476_RS26465
• Protein ID: WP_087444735.1
• Coordinates: 5845608..5845958 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A2W4HCL7
• Locus tag: L3476_RS26460
• Protein ID: WP_040731899.1
• Coordinates: 5845323..5845604 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3476_RS26450 (L3476_26390)	5842521..5843639	+	1119	WP_272826646.1	DUF4367 domain-containing protein	-
L3476_RS26455 (L3476_26395)	5843880..5845100	+	1221	WP_272826647.1	alanine racemase	-
L3476_RS26460 (L3476_26400)	5845323..5845604	+	282	WP_040731899.1	CopG family ribbon-helix-helix protein	Antitoxin
L3476_RS26465 (L3476_26405)	5845608..5845958	+	351	WP_087444735.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L3476_RS26470 (L3476_26410)	5847193..5849664	-	2472	WP_272826648.1	M60 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12862.87 Da
• Isoelectric Point: 5.6312

>T239830 WP_087444735.1 NZ_CP094446:5845608-5845958 [Paenibacillus thiaminolyticus]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEETMRKVDDGLLISLGLIDF

Nucleotide

• Length: 351 bp

>T239830 NZ_LS483350:861708-862001 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAAGTGTACTTAAAAGAGTTCGGTGAACTTATACAAAAGTTT
CATGAAATAGAAAAAGCGTCACTTCAAGCCGACCAAAGCGAAAGTAACGCATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10735.45 Da
• Isoelectric Point: 8.4934

>AT239830 WP_040731899.1 NZ_CP094446:5845323-5845604 [Paenibacillus thiaminolyticus]
VANIQNTKRIMISLPDQLLQEVDGIVAMEKSNRSEFIRQAMKLYLQERKKRHIRESMQRGYMEMAKINLTMASEAFYAEV
EADGTLDRLVSGV

Nucleotide

• Length: 282 bp

>AT239830 NZ_LS483350:861496-861705 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAACTTGGAAAGTTA
TGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2W4HCL7