Detailed information of TA system TA023982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 995673..996199
• Replicon: chromosome
• Accession: NC_012917
• Organism: Pectobacterium carotovorum subsp. carotovorum PC1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: C6DA96
• Locus tag: PC1_RS04265
• Protein ID: WP_012773545.1
• Coordinates: 995673..995978 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6DA97
• Locus tag: PC1_RS04270
• Protein ID: WP_012773546.1
• Coordinates: 995981..996199 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PC1_RS04255	990685..993318	-	2634	WP_012773542.1	DEAD/DEAH box helicase family protein	-
PC1_RS04260	993309..995069	-	1761	WP_012773543.1	site-specific DNA-methyltransferase	-
PC1_RS04265	995673..995978	-	306	WP_012773545.1	type II toxin-antitoxin system toxin CcdB	Toxin
PC1_RS04270	995981..996199	-	219	WP_012773546.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
PC1_RS04275	996258..997001	-	744	WP_012773547.1	hypothetical protein	-
PC1_RS04280	997746..998087	-	342	WP_012773548.1	hypothetical protein	-
PC1_RS04285	998273..998467	+	195	WP_012773549.1	DUF3950 domain-containing protein	-
PC1_RS04290	998562..999821	-	1260	WP_012773550.1	tyrosine-type recombinase/integrase	-
PC1_RS04295	1000254..1000970	-	717	WP_012773552.1	M48 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	983642..1013205	29563

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11762.63 Da
• Isoelectric Point: 4.6627

>T23982 WP_012773545.1 NC_012917:c995978-995673 [Pectobacterium carotovorum subsp. carotovorum PC1]
MQFIVYQYKRASHYTMFVDVQSDIVETPKRRMVIPLIEAHHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVEVIGEVI
TDLGEYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T23982 NC_012917:c995978-995673 [Pectobacterium carotovorum subsp. carotovorum PC1]
ATGCAATTCATTGTTTATCAATACAAACGAGCCAGCCACTACACAATGTTTGTCGATGTGCAGAGTGATATTGTCGAGAC
ACCTAAGCGACGCATGGTCATTCCGCTAATCGAAGCGCATCACCTGTCTGAGAAAGTGAATAAAACGCTGTTTCCTCTGA
TTCGCATCGATGGTGAGGATTATCGGCTGATGACAACTGAACTATCGAGCGTTCCCGTTGAAGTCATTGGTGAAGTGATT
ACGGATCTTGGCGAGTACGCGGATGAGATCAAGGATGCCATTAATCTTATGTTCTGGGGGATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8200.22 Da
• Isoelectric Point: 7.2041

>AT23982 WP_012773546.1 NC_012917:c996199-995981 [Pectobacterium carotovorum subsp. carotovorum PC1]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNEAIGKEARRIKAEEWKKENREGMEEVARFIALHGSFADENRNW

Nucleotide

• Length: 219 bp

>AT23982 NC_012917:c996199-995981 [Pectobacterium carotovorum subsp. carotovorum PC1]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAATTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
GGTGAATGAGGCCATTGGCAAAGAAGCCCGCCGCATCAAGGCTGAGGAATGGAAGAAGGAAAACCGTGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCACTGCATGGCTCTTTTGCGGATGAAAACAGGAACTGGTGA

Structures

Toxin

• AlphaFold DB: C6DA96

Antitoxin

• AlphaFold DB: C6DA97