Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 4821..5422 Replicon plasmid unnamed9
Accession NZ_CP094394
Organism Lactiplantibacillus plantarum subsp. plantarum strain M19

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag KMY87_RS16845 Protein ID WP_001748110.1
Coordinates 4821..5165 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag KMY87_RS16850 Protein ID WP_001748109.1
Coordinates 5159..5422 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KMY87_RS16815 (KMY87_16815) 25..371 - 347 Protein_0 transposase -
KMY87_RS16820 (KMY87_16820) 770..1432 + 663 WP_003645001.1 amino acid ABC transporter permease -
KMY87_RS16825 (KMY87_16825) 1413..2102 + 690 WP_003645002.1 amino acid ABC transporter permease -
KMY87_RS16830 (KMY87_16830) 2095..2835 + 741 WP_015639667.1 amino acid ABC transporter ATP-binding protein -
KMY87_RS16835 (KMY87_16835) 2856..3710 + 855 WP_021816930.1 transporter substrate-binding domain-containing protein -
KMY87_RS16845 (KMY87_16845) 4821..5165 - 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KMY87_RS16850 (KMY87_16850) 5159..5422 - 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
KMY87_RS16855 (KMY87_16855) 5508..6095 + 588 WP_003646144.1 site-specific integrase -
KMY87_RS16860 (KMY87_16860) 8628..9152 - 525 WP_003646143.1 hypothetical protein -
KMY87_RS16865 (KMY87_16865) 9727..9969 - 243 WP_003646142.1 hypothetical protein -
KMY87_RS16870 (KMY87_16870) 10056..10256 - 201 WP_003643340.1 cold-shock protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..13244 13244
- flank IS/Tn - - 3800..4138 338


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T239805 WP_001748110.1 NZ_CP094394:c5165-4821 [Lactiplantibacillus plantarum subsp. plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T239805 NZ_LS483325:c1573662-1572892 [Streptococcus equi subsp. zooepidemicus]
ATGCGGTTAGAAGAATTTAGTCAAGATGACTTTGACCTAGCCTTAAAACGAACCATTCGTTCTTTAACTCGTGGTAAAAC
AACATCAGTTAGTCCAAAGGCAATTTTACTTGGTGGACAAAGCGGAGCAGGAAAAACAACGATACACCGTATCGAGCAAA
AAGAGTTTCAAGGGAATATCATTATTATTGACGGCGATAGCTATCGTTCTTTACACCCGAATTATCTAGCTTTACACGAA
GAATACGGTAAGGATAGCGTGGATTATACCAAAGGTTTTGCAGGAAAAATGGTGGAACATCTTGTTGATGATTTAAGTAA
ACAAGGTTATCATTTGTTAATTGAAGGAACCTTGAGAACGACAGAAGTCCCACGAAAAACAGCTCGGTTATTGAATTCGA
GAGGTTATCAAGTCTCTTTAGCTTTGATAGCGACCAAGCCTGAATTGTCATATCTCAGTACCTTAATCCGCTATGAGGAA
GTCTATGCGGTGAACCCAAACCAGGCTAGAGCAACTCCTAAAGAACATCACGATGGTATTGTAGACCATTTAGTCGATAA
TCTAAGAGAGTTAGAAAACGATAAACTCTTTGGCCAGATTCAAATATATCAAAGAGATAGAACCTGTGTCTATGATTCAA
AGACGGATGAAGGTTCAGCAGCCGATGTTCTACAAGAATGCCTCTTCGGAAAATGGAGTAAGGTCGAGGAAGAGATGTTG
AAGGTGGGACAAGAATGTTTGAGGGAGTTAGAGTTTAAAGGAGAGTGCTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT239805 WP_001748109.1 NZ_CP094394:c5422-5159 [Lactiplantibacillus plantarum subsp. plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT239805 NZ_LS483325:c1574138-1573662 [Streptococcus equi subsp. zooepidemicus]
ATGATTGGAGACAATATCAAATCGTTACGTAAAACCCATGATTTAACACAACCAGAATTCGCAAAGATTGTGGGAATTTC
ACGAAATAGCCTTAGTCGTTATGAAAATGGAACCAGCTCTGTTTCAACAGAACTGATAGATCGTATTTGTCAGAAATTCA
ACGTGTCTTATGTCGACATTGTAGGAGAAGAAAAGCTGTTAACACCAGTAGAAGATTATCAATTGACGCTGAAAATCGAA
GTCATCAAAGAACGTGGTGCAGCGATTTTATCCGAACTGTATTGCTATCAAGATAGTCAAGGAATTGCTTTTGATGATGA
GACTAGCCCTTGGATTTTGATGAGTGATGACTTGTCAGACCTTATCAACACTAAGATTTACCTTGTCAATACCTTTGACG
AAGTAGAGCGCTACAATGGTTATTTGGATGGCATTGAGCGTATGTTGGAGCTTGCTCGACATCAGGTGGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References