Detailed information of TA system TA239779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2526602..2527147
• Replicon: chromosome
• Accession: NZ_CP094359
• Organism: Acidithiobacillus sp. YTS05

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: MQE22_RS12550
• Protein ID: WP_226827702.1
• Coordinates: 2526896..2527147 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MQE22_RS12545
• Protein ID: WP_215872074.1
• Coordinates: 2526602..2526892 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQE22_RS12520 (MQE22_12515)	2523362..2524081	+	720	WP_215872070.1	TIGR00730 family Rossman fold protein	-
MQE22_RS12525 (MQE22_12520)	2524100..2524432	+	333	WP_215872071.1	DUF2782 domain-containing protein	-
MQE22_RS12530 (MQE22_12525)	2524401..2525369	+	969	WP_226827699.1	homoserine kinase	-
MQE22_RS12535 (MQE22_12530)	2525382..2525690	-	309	WP_215872072.1	hypothetical protein	-
MQE22_RS12540 (MQE22_12535)	2525789..2526376	+	588	WP_215872073.1	cytochrome b/b6 domain-containing protein	-
MQE22_RS12545 (MQE22_12540)	2526602..2526892	-	291	WP_215872074.1	putative addiction module antidote protein	Antitoxin
MQE22_RS12550 (MQE22_12545)	2526896..2527147	-	252	WP_226827702.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MQE22_RS12555 (MQE22_12550)	2527351..2527680	+	330	WP_215872075.1	BrnT family toxin	-
MQE22_RS12560 (MQE22_12555)	2527652..2527990	+	339	WP_226827704.1	BrnA antitoxin family protein	-
MQE22_RS12565 (MQE22_12560)	2528013..2528273	-	261	WP_226827707.1	hypothetical protein	-
MQE22_RS12570 (MQE22_12565)	2528266..2528529	-	264	WP_215872076.1	Txe/YoeB family addiction module toxin	-
MQE22_RS12575 (MQE22_12570)	2528526..2528768	-	243	WP_215872077.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MQE22_RS12580 (MQE22_12575)	2528907..2530271	-	1365	WP_226827711.1	group II intron reverse transcriptase/maturase	-
MQE22_RS12585 (MQE22_12580)	2530643..2531074	-	432	WP_226827737.1	methyltransferase domain-containing protein	-
MQE22_RS12590 (MQE22_12585)	2531227..2531598	-	372	WP_215872078.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-79)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9444.85 Da
• Isoelectric Point: 10.0968

>T239779 WP_226827702.1 NZ_CP094359:c2527147-2526896 [Acidithiobacillus sp. YTS05]
MKDKVDRARIAHRIRSAEHGNFGDCEPVGEGVSEMRVHVGPGYRVYYTRRGVVVYLLLLAGDKSSQKRDIKRAIEMARSL
DKE

Nucleotide

• Length: 252 bp

>T239779 NZ_LS483317:c2479163-2478897 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10371.92 Da
• Isoelectric Point: 5.0745

>AT239779 WP_215872074.1 NZ_CP094359:c2526892-2526602 [Acidithiobacillus sp. YTS05]
MAKLAPFDAADYLDDEETIAEFLTAALEDQNPDVFLSAVRDVARARGMAQLAKDAGLGRESLYKALTPGAKPRYDTMLKL
LHALGVKLSATPLIHS

Nucleotide

• Length: 291 bp

>AT239779 NZ_LS483317:c2479414-2479163 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCTATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA