Detailed information of TA system TA023977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 1288829..1289464
• Replicon: chromosome
• Accession: NC_012914
• Organism: Paenibacillus sp. JDR-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: C6CVP0
• Locus tag: PJDR2_RS05580
• Protein ID: WP_015842701.1
• Coordinates: 1289114..1289464 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: PJDR2_RS05575
• Protein ID: WP_041613299.1
• Coordinates: 1288829..1289110 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PJDR2_RS05565	1286030..1287259	+	1230	WP_015842698.1	outer membrane lipoprotein carrier protein LolA	-
PJDR2_RS05570	1287427..1288611	+	1185	WP_015842699.1	alanine racemase	-
PJDR2_RS05575	1288829..1289110	+	282	WP_041613299.1	CopG family ribbon-helix-helix protein	Antitoxin
PJDR2_RS05580	1289114..1289464	+	351	WP_015842701.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PJDR2_RS05585	1289719..1290432	+	714	WP_015842702.1	SAF domain-containing protein	-
PJDR2_RS05595	1291004..1292641	+	1638	WP_150106442.1	CpaF family protein	-
PJDR2_RS05600	1292641..1293573	+	933	WP_150106615.1	type II secretion system F family protein	-
PJDR2_RS05605	1293593..1294459	+	867	WP_015842706.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12779.76 Da
• Isoelectric Point: 5.1284

>T23977 WP_015842701.1 NC_012914:1289114-1289464 [Paenibacillus sp. JDR-2]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEMEAAAHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDDETMRKVDDALLISVGLIDF

Nucleotide

• Length: 351 bp

>T23977 NC_012914:1289114-1289464 [Paenibacillus sp. JDR-2]
TTGATCGTAAAACGCGGAGATGTGTTTTTTGCGGATTTGTCACCAGTCGTCGGATCGGAACAGGGCGGAGTAAGACCCGT
ATTAGTTATTCAGAATGATATCGGCAACCGCTTCAGTCCGACGGTTATCGTCGCGGCCATTACGGCGCAAATCCAGAAAG
CCAAATTGCCTACGCACGTTGAAATGGAAGCGGCGGCTCACGGCTTCGACAGAGACTCCGTCGTGCTGCTTGAGCAGATT
AGGACGATTGACAAGCAGCGTTTAACCGACAAGATCACCCACCTGGACGATGAAACGATGCGTAAAGTGGACGATGCTCT
GCTGATCAGCGTTGGATTAATCGATTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10711.33 Da
• Isoelectric Point: 7.0158

>AT23977 WP_041613299.1 NC_012914:1288829-1289110 [Paenibacillus sp. JDR-2]
VANMQNTKRIMISLPDQLLEEVDGIVAKENSNRSEFIRQAMKLYLVERKKRQIRESMQRGYLEMAKINLNMASEAFQAEE
EADHTLGRLVSGV

Nucleotide

• Length: 282 bp

>AT23977 NC_012914:1288829-1289110 [Paenibacillus sp. JDR-2]
GTGGCCAATATGCAGAACACCAAGAGGATCATGATCAGCTTGCCGGATCAATTGCTGGAGGAGGTCGACGGCATCGTCGC
CAAGGAAAATTCCAACCGCAGCGAGTTTATTCGGCAGGCCATGAAGCTGTATCTTGTGGAACGAAAAAAACGGCAGATCC
GTGAGTCCATGCAGCGTGGGTATCTGGAAATGGCGAAGATCAACTTGAACATGGCCTCCGAAGCTTTTCAAGCGGAGGAA
GAAGCCGATCATACGTTAGGCCGTCTCGTAAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A1I2HGR7