Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 6182389..6183008 Replicon chromosome
Accession NZ_CP094343
Organism Pseudomonas sp. M1

Toxin (Protein)


Gene name hicA Uniprot ID W5J3G4
Locus tag PM1_RS27515 Protein ID WP_009615522.1
Coordinates 6182826..6183008 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID W5J3H2
Locus tag PM1_RS27510 Protein ID WP_009615524.1
Coordinates 6182389..6182793 (-) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PM1_RS27490 (MRY70_27490) 6177912..6179873 + 1962 WP_202964458.1 restriction endonuclease subunit R -
PM1_RS27495 (MRY70_27495) 6180356..6181541 + 1186 Protein_5421 Fic family protein -
PM1_RS27500 (MRY70_27500) 6181562..6181986 - 425 Protein_5422 type II toxin-antitoxin system VapC family toxin -
PM1_RS27505 (MRY70_27505) 6181986..6182240 - 255 WP_009615526.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
PM1_RS27510 (MRY70_27510) 6182389..6182793 - 405 WP_009615524.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
PM1_RS27515 (MRY70_27515) 6182826..6183008 - 183 WP_009615522.1 type II toxin-antitoxin system HicA family toxin Toxin
PM1_RS27520 (MRY70_27520) 6183467..6183955 + 489 WP_009615520.1 BRO family protein -
PM1_RS27525 (MRY70_27525) 6184256..6184414 - 159 WP_009615518.1 YqaE/Pmp3 family membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - rpe 6110434..6183955 73521


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6962.13 Da        Isoelectric Point: 12.2993

>T239755 WP_009615522.1 NZ_CP094343:c6183008-6182826 [Pseudomonas sp. M1]
MRSREIMELIRADGWFLVEVKGSHHQFRHFTKKGRVTVPHPRSNLPIGTVNSILRQAGLR

Download         Length: 183 bp

>T239755 NZ_LS483314:c2102469-2102107 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14532.23 Da        Isoelectric Point: 4.5714

>AT239755 WP_009615524.1 NZ_CP094343:c6182793-6182389 [Pseudomonas sp. M1]
MKFPVVLHKDADSDYGVTIPDVPGCFSAGGTVSQALENVQEALALHFEGLVADGETLPQAQEVDTHMGNPDYAGGVWAVV
DFDVTPYLGKAVRFNASLPENLLQRIDERVKRDHRYASRSGFLATAALRELSSN

Download         Length: 405 bp

>AT239755 NZ_LS483314:c2102636-2102466 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A127MLP4

Antitoxin

Source ID Structure
AlphaFold DB A0A1A5CY34

References