Detailed information of TA system TA239753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 5765567..5766156
• Replicon: chromosome
• Accession: NZ_CP094343
• Organism: Pseudomonas sp. M1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: W5IST2
• Locus tag: PM1_RS25475
• Protein ID: WP_009617642.1
• Coordinates: 5765905..5766156 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: W5ITY9
• Locus tag: PM1_RS25470
• Protein ID: WP_009617644.1
• Coordinates: 5765567..5765908 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PM1_RS25450 (MRY70_25450)	5762489..5763337	+	849	WP_009617652.1	carboxylating nicotinate-nucleotide diphosphorylase	-
PM1_RS25455 (MRY70_25455)	5763639..5763890	+	252	WP_009617650.1	helix-turn-helix domain-containing protein	-
PM1_RS25460 (MRY70_25460)	5763890..5765086	+	1197	WP_009617648.1	type II toxin-antitoxin system HipA family toxin	-
PM1_RS25465 (MRY70_25465)	5765274..5765501	-	228	WP_009617646.1	DUF433 domain-containing protein	-
PM1_RS25470 (MRY70_25470)	5765567..5765908	-	342	WP_009617644.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PM1_RS25475 (MRY70_25475)	5765905..5766156	-	252	WP_009617642.1	type II toxin-antitoxin system HicA family toxin	Toxin
PM1_RS25480 (MRY70_25480)	5766260..5766406	+	147	WP_024128154.1	hypothetical protein	-
PM1_RS25485 (MRY70_25485)	5766403..5767476	-	1074	WP_009617639.1	diguanylate cyclase	-
PM1_RS25495 (MRY70_25495)	5767752..5769305	-	1554	WP_009617637.1	O-antigen ligase family protein	-
PM1_RS25500 (MRY70_25500)	5769319..5771154	-	1836	WP_161788510.1	sulfatase-like hydrolase/transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	pilB / pilC / xcpA/pilD	5763639..5777553	13914

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9535.00 Da
• Isoelectric Point: 10.0940

>T239753 WP_009617642.1 NZ_CP094343:c5766156-5765905 [Pseudomonas sp. M1]
MSRNEKLLAKLLNESASFTWQELVTLLRWLGYRQLEGSGSRVKFDNGDPQALINLHKPHPGNELKAYVRRQIIEHLKAGE
LIP

Nucleotide

• Length: 252 bp

>T239753 NZ_LS483314:c1929300-1929148 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12644.06 Da
• Isoelectric Point: 4.8281

>AT239753 WP_009617644.1 NZ_CP094343:c5765908-5765567 [Pseudomonas sp. M1]
VNNLLQYRGYYGSIEASTEDNCLFGKLLFIRALVNYEGETVAELQAAFREAVDDYLATCQAQGQEPEVPCKGSFNVRVGH
DLHLAASLAANRQHMSLNDLTRQALSEYLQHHD

Nucleotide

• Length: 342 bp

>AT239753 NZ_LS483314:c1929924-1929325 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGACACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: W5IST2

Antitoxin

• AlphaFold DB: W5ITY9