Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 5238207..5238850 Replicon chromosome
Accession NZ_CP094343
Organism Pseudomonas sp. M1

Toxin (Protein)


Gene name vapC Uniprot ID W5ISZ1
Locus tag PM1_RS23020 Protein ID WP_009620640.1
Coordinates 5238437..5238850 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID W5IS21
Locus tag PM1_RS23015 Protein ID WP_009620639.1
Coordinates 5238207..5238437 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PM1_RS22995 (MRY70_22995) 5234435..5235355 + 921 WP_009620635.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
PM1_RS23000 (MRY70_23000) 5235352..5236605 + 1254 WP_009620636.1 high-affinity branched-chain amino acid ABC transporter permease LivM -
PM1_RS23005 (MRY70_23005) 5236602..5237369 + 768 WP_009620637.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
PM1_RS23010 (MRY70_23010) 5237373..5238074 + 702 WP_009620638.1 ATP-binding cassette domain-containing protein -
PM1_RS23015 (MRY70_23015) 5238207..5238437 + 231 WP_009620639.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
PM1_RS23020 (MRY70_23020) 5238437..5238850 + 414 WP_009620640.1 tRNA(fMet)-specific endonuclease VapC Toxin
PM1_RS23025 (MRY70_23025) 5239338..5240027 - 690 WP_009620641.1 GntR family transcriptional regulator -
PM1_RS23030 (MRY70_23030) 5240092..5240826 - 735 WP_009620642.1 SDR family oxidoreductase -
PM1_RS23035 (MRY70_23035) 5240979..5242043 + 1065 WP_009620643.1 phosphotransferase -
PM1_RS23040 (MRY70_23040) 5242040..5243362 + 1323 WP_009620644.1 aminotransferase class III-fold pyridoxal phosphate-dependent enzyme -
PM1_RS23045 (MRY70_23045) 5243375..5243833 - 459 WP_009620645.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15167.46 Da        Isoelectric Point: 6.9789

>T239750 WP_009620640.1 NZ_CP094343:5238437-5238850 [Pseudomonas sp. M1]
MLKFMLDTNICIFTIKNKPQVVREAFNQHHGQLAISSVTLMELIYGAEKSSMPEHNLGIVEGFAARLEVLDYDGNAAAHT
GQLRAELARIGKPIGPYDQMIAGHARSRGLILVSNNLREFERVPGLRLEDWTAAPSP

Download         Length: 414 bp

>T239750 NZ_LS483313:1038224-1038430 [Staphylococcus simulans]
ATGCATAGTAGTGTGATTCAAATCAGTGGTATGGAAACTGAAGAACAACAGCATATTTTGCAAGAACATTTGCAGCAAAT
GATTGGTATGTCAGAAGTGCAAGTGGATCTGAAATCACAAGAAGTGAAAGCGAGTTACCAGACACCAATCAGTTTAAACA
ATTTGGAAAAAGAAATTTATGATCAAGGTTACCAAATATTAAATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.43 Da        Isoelectric Point: 4.3094

>AT239750 WP_009620639.1 NZ_CP094343:5238207-5238437 [Pseudomonas sp. M1]
MEQGAVFQSNRSQAVRLPKAVALPEDVKRVDVVAIGRTRILTPAGESWDSWFDGENVSADFMSEREQPAEQEREGF

Download         Length: 231 bp

>AT239750 NZ_LS483313:1037916-1038209 [Staphylococcus simulans]
ATGACGCAAGAAGAACGCGCACATCATTCAGAAAAAATAAAATCTAACCTTAAAGCACGACTGAATCGTATAGAAGGACA
AGTCAGAGCGATTAATCGCATGATAGATGAAGATGTTTATTGTGATGATGTTTTAACACAAATCAGAGCGACACGTTCAG
CTTTGAATAGTGTTGCTGTAAAATTGCTGGATCATCATATGAAAGGCTGTATTTTGCATAAAGCTGAAGATGGACAAGCA
GAAGCTGCAATGGAAGAATTATTAGTTACCGTCCAAAAATTAATTAAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W5ISZ1

Antitoxin

Source ID Structure
AlphaFold DB A0A1A5DDH0

References