Detailed information of TA system TA023975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1238884..1240442
• Replicon: chromosome
• Accession: NC_012913
• Organism: Aggregatibacter aphrophilus NJ8700

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: NT05HA_RS05615
• Protein ID: WP_012771640.1
• Coordinates: 1239162..1240442 (+)
• Length: 427 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0M3G8E2
• Locus tag: NT05HA_RS05610
• Protein ID: WP_012771639.1
• Coordinates: 1238884..1239162 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NT05HA_RS05590	1234058..1234540	-	483	WP_012771635.1	DNA starvation/stationary phase protection protein	-
NT05HA_RS05595	1234731..1235891	+	1161	WP_012771636.1	EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit	-
NT05HA_RS05600	1235904..1237418	+	1515	WP_012771637.1	DHA2 family efflux MFS transporter permease subunit	-
NT05HA_RS05605	1237675..1238730	+	1056	WP_012771638.1	ABC transporter substrate-binding protein	-
NT05HA_RS05610	1238884..1239162	+	279	WP_012771639.1	helix-turn-helix domain-containing protein	Antitoxin
NT05HA_RS05615	1239162..1240442	+	1281	WP_012771640.1	type II toxin-antitoxin system HipA family toxin	Toxin
NT05HA_RS05620	1240525..1243389	-	2865	WP_012771641.1	valine--tRNA ligase	-
NT05HA_RS05625	1243445..1243984	-	540	WP_005700993.1	hypothetical protein	-
NT05HA_RS05630	1243977..1244303	-	327	WP_005700994.1	N(4)-acetylcytidine aminohydrolase	-
NT05HA_RS05635	1244381..1244827	-	447	WP_005700996.1	DNA polymerase III subunit chi	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (6-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-63)

Sequences

Toxin

Protein

• Length: 427 a.a.
• Molecular weight: 49061.25 Da
• Isoelectric Point: 7.7671

>T23975 WP_012771640.1 NC_012913:1239162-1240442 [Aggregatibacter aphrophilus NJ8700]
MSNSILNVAMNGLLVGEWRKLTSGATEFQYAEKWLESVRSRPISLSLPLSHKIYRGDSVYNFFDNLLPDNEQIRSRIQQR
FQTSTKSPFDLLSAIGQDCIGAIQLYQGESRSVRQIYAEPLNDKEVENVLKNYRTFPLGMEESEDFRISLAGAQEKTALL
YYQNQWQRPLHSTATSHIFKLPIGMVAQGQLDLSGSCENEWLCVNIMKAFGLPVPRSQIQYFGEMKVLIVERFDRRWSEN
RSWLIRLPQEDMCQALNIAPARKYENDGGPSILQTMSLLNGSSNAQADRKQFLKAQIVFWLLCAIDGHGKNFSLFLEQEN
RYRLTPFYDVMSAYPLIKHNGLQKQKVKMAMAWHGENKHYLWDKIQLRHIFNTAKLAGLAKETVEDILHEISSLYPRVNE
INTHGLPDEIVDPIFMGLEMQMKKIS

Nucleotide

• Length: 1281 bp

>T23975 NC_012913:1239162-1240442 [Aggregatibacter aphrophilus NJ8700]
ATGAGTAACTCGATTCTAAACGTTGCCATGAATGGCTTGTTAGTCGGTGAATGGCGAAAACTCACCAGTGGCGCAACCGA
ATTTCAGTATGCGGAAAAATGGCTTGAATCTGTTCGCTCGCGCCCTATTTCATTGTCACTGCCCTTGTCGCACAAAATTT
ATCGAGGAGATTCCGTTTACAATTTCTTCGATAATCTCCTCCCCGACAACGAACAGATCCGTTCACGTATTCAACAACGT
TTCCAAACCTCAACGAAATCCCCATTTGATTTACTTTCGGCGATTGGACAGGATTGTATCGGCGCAATTCAGCTTTATCA
GGGCGAGAGCCGCTCGGTACGTCAAATTTATGCAGAACCACTAAATGACAAAGAGGTGGAAAACGTGCTGAAAAACTACC
GCACTTTTCCTCTCGGCATGGAAGAAAGCGAGGATTTCCGAATTTCTTTAGCCGGCGCTCAGGAAAAAACAGCCCTCCTT
TATTACCAAAATCAATGGCAGCGCCCTTTGCATTCCACCGCGACCAGCCATATTTTTAAACTCCCGATTGGCATGGTGGC
ACAAGGGCAACTTGATCTTTCCGGCAGCTGTGAAAATGAATGGCTTTGCGTGAATATTATGAAAGCCTTCGGTTTGCCGG
TTCCTCGAAGCCAAATTCAGTATTTCGGTGAAATGAAAGTACTGATTGTGGAGCGCTTTGATCGCCGTTGGTCTGAAAAT
AGGAGTTGGCTAATTCGTTTGCCACAGGAAGATATGTGCCAAGCACTCAACATCGCTCCTGCACGAAAATATGAAAATGA
CGGTGGTCCGAGCATTCTTCAAACTATGTCGCTCCTCAACGGTTCATCCAATGCCCAAGCAGATCGCAAACAGTTTCTTA
AAGCGCAAATTGTTTTTTGGCTGCTTTGTGCTATTGACGGCCATGGAAAAAATTTCAGTTTGTTTTTAGAACAAGAGAAC
CGCTATCGTCTAACGCCTTTTTATGACGTGATGTCCGCCTATCCGCTCATCAAACACAACGGACTACAAAAGCAAAAAGT
TAAAATGGCAATGGCGTGGCACGGCGAAAATAAACATTATTTATGGGATAAAATACAACTCCGCCACATTTTTAATACTG
CTAAACTCGCCGGCTTGGCAAAGGAAACTGTAGAAGACATTTTGCATGAAATCAGTAGCCTATACCCAAGAGTAAATGAA
ATAAATACCCATGGATTGCCTGATGAAATTGTCGATCCTATTTTCATGGGATTGGAAATGCAGATGAAAAAGATAAGTTA
A

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10414.83 Da
• Isoelectric Point: 5.1651

>AT23975 WP_012771639.1 NC_012913:1238884-1239162 [Aggregatibacter aphrophilus NJ8700]
MVITTAKMLSHVIREFRYQGDLSQTDVAKLAGTTQARISAFENEPERTKLETLFKILAGLELELIVQPRKKSSDTARSIK
EPTATYDDDEAW

Nucleotide

• Length: 279 bp

>AT23975 NC_012913:1238884-1239162 [Aggregatibacter aphrophilus NJ8700]
ATGGTTATCACCACCGCAAAAATGCTTTCTCATGTAATTCGGGAATTTCGCTATCAAGGCGATCTGTCACAAACTGATGT
TGCTAAACTCGCCGGAACTACCCAAGCTAGAATTTCAGCGTTTGAGAATGAGCCGGAGCGCACTAAATTAGAAACACTAT
TTAAAATTCTGGCTGGACTAGAACTAGAGCTTATTGTGCAACCACGCAAAAAGTCATCTGATACTGCTCGCTCAATCAAG
GAGCCAACAGCAACATACGATGACGATGAGGCATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M3G8E2