Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 4542455..4543044 Replicon chromosome
Accession NZ_CP094343
Organism Pseudomonas sp. M1

Toxin (Protein)


Gene name pemK Uniprot ID W5IWI7
Locus tag PM1_RS19860 Protein ID WP_009618988.1
Coordinates 4542688..4543044 (+) Length 119 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID W5IWC6
Locus tag PM1_RS19855 Protein ID WP_009618989.1
Coordinates 4542455..4542694 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PM1_RS19830 (MRY70_19830) 4537909..4538370 + 462 WP_169720932.1 hypothetical protein -
PM1_RS19835 (MRY70_19835) 4538404..4539111 + 708 WP_009618993.1 peptidoglycan-binding domain-containing protein -
PM1_RS19840 (MRY70_19840) 4539126..4540745 + 1620 WP_009618992.1 hypothetical protein -
PM1_RS19845 (MRY70_19845) 4540826..4541662 + 837 WP_009618991.1 AraC family transcriptional regulator -
PM1_RS19850 (MRY70_19850) 4541714..4542319 + 606 WP_009618990.1 LysE family translocator -
PM1_RS19855 (MRY70_19855) 4542455..4542694 + 240 WP_009618989.1 antitoxin PemI Antitoxin
PM1_RS19860 (MRY70_19860) 4542688..4543044 + 357 WP_009618988.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
PM1_RS19865 (MRY70_19865) 4543519..4544328 - 810 WP_009618987.1 class I SAM-dependent methyltransferase -
PM1_RS19870 (MRY70_19870) 4544420..4545013 - 594 WP_009618986.1 histidine phosphatase family protein -
PM1_RS19875 (MRY70_19875) 4545251..4545679 - 429 WP_009618985.1 hypothetical protein -
PM1_RS19880 (MRY70_19880) 4546103..4546453 + 351 WP_009618984.1 hypothetical protein -
PM1_RS19885 (MRY70_19885) 4546475..4547569 - 1095 WP_009618983.1 D-alanine--D-alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-117)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13133.98 Da        Isoelectric Point: 7.3242

>T239749 WP_009618988.1 NZ_CP094343:4542688-4543044 [Pseudomonas sp. M1]
MVRRPPSVPDRGEIWHLQFDPSTGREMIGDHFCLVLSKREFNEMGLALVCPISQGASNVARNQGFLVTLMGEGTDTQGNV
HSHQVRTLDWRARRAAFKEKAPDSVTNYVLSLVGAILE

Download         Length: 357 bp

>T239749 NZ_LS483312:1112151-1112321 [Staphylococcus lugdunensis]
ATGTCAAAAGAAAAAAAGAGTCGTTTAAATTATCATGAGGAAGAGAATGCGATGGTTGAAAATTTAGAAGACTTAAAAGA
GCTGGGCAAAGAAATGGAACACATCTCAGAGAAAAATGACGAAGAAAAAGTTAATCAATCGCATGATACATCAAAGTCTT
CAAATTCATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8345.65 Da        Isoelectric Point: 4.8668

>AT239749 WP_009618989.1 NZ_CP094343:4542455-4542694 [Pseudomonas sp. M1]
MTTLALRKSGGSLIISVPSAFVAQNKLHAGSQVECDIVEGALVIKPMTRPSLAQLIAETPKDSLAPDWDSMPAVGAEQW

Download         Length: 240 bp

>AT239749 NZ_LS483312:1111512-1112111 [Staphylococcus lugdunensis]
ATGGCAATGAACTTTAAAGTATTTGAAAATAAAGATCAAGTTGCAGAGTATTCAGCAGATATCATTAGAAAACAATTTAA
CAATAATCCTACTACAATTGCTGGATTTCATTTAAACAAAGAGGCATCACCAGTACTTGATATGCTTAAAAAGAATGTTG
ACCAACATGCGGTTGACTTCAGCCAAATTAACATTCTTGACTATGATCAAAATCAAGAATATTTCAAAGCGCTGGGGGTT
CCTGAGAGTCAAATTTATCCTATCTCATACGATCAAGATGCTGAAGCGTTTATCTCTGATAAAATTAAAACAAAAGATAA
TAAAGGGAAACTAATATTACAAGTAGCTTCAATTGATGATCAAGGAAACCTTAATATCAGTCTACGTCAAGGATTGTTAA
ATGCTAGAGAAATTATCTTAGTCATTACAGGACATAATAAAAAAGAAGTAGTAGAAAAACTGTATAATGAAAATGGAAAA
TCTAATTTTGAACCTGCTGATTTAAAAGCACATCGTATGGTAACGGTTATTTTAGACGAAGCGGCAGCTGAAGGTTTGCC
AGAAGATGTACGTCATTACTTTACTGATCGGTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W5IWI7

Antitoxin

Source ID Structure
AlphaFold DB A0A1A5D3H0

References