Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4624921..4625418 Replicon chromosome
Accession NZ_CP094339
Organism Escherichia coli strain W224N

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MQV61_RS22710 Protein ID WP_001173343.1
Coordinates 4624921..4625094 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag MQV61_RS22715 Protein ID WP_001312331.1
Coordinates 4625122..4625418 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MQV61_RS22700 (4621591) 4621591..4623048 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
MQV61_RS22705 (4623285) 4623285..4624802 + 1518 WP_000003806.1 lysine--tRNA ligase -
MQV61_RS22710 (4624921) 4624921..4625094 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MQV61_RS22715 (4625122) 4625122..4625418 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MQV61_RS22720 (4625647) 4625647..4625919 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
MQV61_RS22725 (4625931) 4625931..4626161 - 231 WP_000373293.1 4Fe-4S mono-cluster protein YjdI -
MQV61_RS22730 (4626342) 4626342..4627973 + 1632 WP_001216482.1 sensor histidine kinase -
MQV61_RS22735 (4627970) 4627970..4628689 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T239732 WP_001173343.1 NZ_CP094339:c4625094-4624921 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T239732 NZ_LS483310:c2378848-2378582 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT239732 WP_001312331.1 NZ_CP094339:c4625418-4625122 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT239732 NZ_LS483310:c2379099-2378848 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References