Detailed information of TA system TA239720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2556161..2557749
• Replicon: chromosome
• Accession: NZ_CP094339
• Organism: Escherichia coli strain W224N

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: MQV61_RS12500
• Protein ID: WP_001125475.1
• Coordinates: 2556427..2557749 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: MQV61_RS12495
• Protein ID: WP_001332145.1
• Coordinates: 2556161..2556427 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQV61_RS12480 (2552529)	2552529..2553980	+	1452	WP_000854637.1	tagaturonate reductase	-
MQV61_RS12485 (2554177)	2554177..2555091	+	915	WP_001306069.1	bestrophin family protein	-
MQV61_RS12490 (2555095)	2555095..2555853	-	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
MQV61_RS12495 (2556161)	2556161..2556427	+	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
MQV61_RS12500 (2556427)	2556427..2557749	+	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
MQV61_RS12505 (2558059)	2558059..2558241	-	183	Protein_2450	hypothetical protein	-
MQV61_RS12510 (2558375)	2558375..2559157	+	783	WP_001221834.1	SDR family oxidoreductase	-
MQV61_RS12515 (2559401)	2559401..2559508	-	108	Protein_2452	hypothetical protein	-
MQV61_RS12520 (2559933)	2559933..2560127	+	195	Protein_2453	fimbrial protein	-
MQV61_RS12525 (2560101)	2560101..2560799	+	699	WP_166709565.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T239720 WP_001125475.1 NZ_CP094339:2556427-2557749 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T239720 NZ_LS483309:c2136141-2135965 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT239720 WP_001332145.1 NZ_CP094339:2556161-2556427 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT239720 NZ_LS483309:2135842-2135897 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8