Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1091803..1092386 Replicon chromosome
Accession NZ_CP094339
Organism Escherichia coli strain W224N

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag MQV61_RS05215 Protein ID WP_000254750.1
Coordinates 1092051..1092386 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag MQV61_RS05210 Protein ID WP_000581937.1
Coordinates 1091803..1092051 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MQV61_RS05200 (1088142) 1088142..1089443 + 1302 WP_000046816.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
MQV61_RS05205 (1089491) 1089491..1091725 + 2235 WP_000226815.1 GTP pyrophosphokinase -
MQV61_RS05210 (1091803) 1091803..1092051 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MQV61_RS05215 (1092051) 1092051..1092386 + 336 WP_000254750.1 endoribonuclease MazF Toxin
MQV61_RS05220 (1092458) 1092458..1093249 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
MQV61_RS05225 (1093477) 1093477..1095114 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
MQV61_RS05230 (1095202) 1095202..1096500 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T239705 WP_000254750.1 NZ_CP094339:1092051-1092386 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T239705 NZ_LS483308:1836597-1836692 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT239705 WP_000581937.1 NZ_CP094339:1091803-1092051 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT239705 NZ_LS483308:c1836777-1836720 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References