Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 934486..935140 Replicon chromosome
Accession NZ_CP094339
Organism Escherichia coli strain W224N

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag MQV61_RS04565 Protein ID WP_000244781.1
Coordinates 934733..935140 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MQV61_RS04560 Protein ID WP_000354046.1
Coordinates 934486..934752 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MQV61_RS04540 (930574) 930574..932007 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
MQV61_RS04545 (932052) 932052..932363 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
MQV61_RS04550 (932527) 932527..933186 + 660 WP_000250274.1 hemolysin III family protein -
MQV61_RS04555 (933263) 933263..934243 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
MQV61_RS04560 (934486) 934486..934752 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MQV61_RS04565 (934733) 934733..935140 + 408 WP_000244781.1 protein YgfX Toxin
MQV61_RS04570 (935180) 935180..935701 - 522 WP_001055874.1 flavodoxin FldB -
MQV61_RS04575 (935813) 935813..936709 + 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
MQV61_RS04580 (936734) 936734..937444 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MQV61_RS04585 (937450) 937450..939183 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T239704 WP_000244781.1 NZ_CP094339:934733-935140 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T239704 NZ_LS483308:c825760-825572 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT239704 WP_000354046.1 NZ_CP094339:934486-934752 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT239704 NZ_LS483308:c826049-825789 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References