Detailed information of TA system TA023968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4024096..4024880
• Replicon: chromosome
• Accession: NC_012912
• Organism: Dickeya zeae Ech1591

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: C6CKJ1
• Locus tag: DD1591_RS17630
• Protein ID: WP_015847872.1
• Coordinates: 4024096..4024590 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: C6CKJ2
• Locus tag: DD1591_RS17635
• Protein ID: WP_015847873.1
• Coordinates: 4024587..4024880 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DD1591_RS17605	4019629..4020372	+	744	WP_015847867.1	hypothetical protein	-
DD1591_RS17610	4020431..4020649	+	219	WP_015847868.1	type II toxin-antitoxin system antitoxin CcdA	-
DD1591_RS17615	4020652..4020957	+	306	WP_015847869.1	type II toxin-antitoxin system toxin CcdB	-
DD1591_RS17620	4021156..4021998	-	843	WP_015847870.1	DNA adenine methylase	-
DD1591_RS17625	4022084..4023805	-	1722	WP_015847871.1	AAA family ATPase	-
DD1591_RS17630	4024096..4024590	-	495	WP_015847872.1	GNAT family N-acetyltransferase	Toxin
DD1591_RS17635	4024587..4024880	-	294	WP_015847873.1	DUF1778 domain-containing protein	Antitoxin
DD1591_RS17640	4025405..4025905	-	501	WP_015847874.1	serine acetyltransferase	-
DD1591_RS17645	4025932..4027326	-	1395	WP_015847875.1	DUF4832 domain-containing protein	-
DD1591_RS17650	4027365..4027772	-	408	WP_015847876.1	membrane protein	-
DD1591_RS17655	4027791..4028903	-	1113	WP_015847877.1	glycosyltransferase family 4 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4016488..4025136	8648

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (76-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17568.39 Da
• Isoelectric Point: 7.2922

>T23968 WP_015847872.1 NC_012912:c4024590-4024096 [Dickeya chrysanthemi Ech1591]
MISAPEPLHAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDGSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDESLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEARAFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T23968 NC_012912:c4024590-4024096 [Dickeya chrysanthemi Ech1591]
ATGATCTCTGCCCCTGAGCCACTCCACGCCGAACATGTACTTTCCTCATTCTGTTGCGGCGTGGAGTCTATGGACAACTG
GCTGAAACAACGGGCGATGAAAAATCAGGTCACTGGTGCATCACGAACTTTCGTCAGTTGCGATGGCTCAAAAGTGTTGG
CGTATTACTCATTGGCTTCCAGTGCTGTGGCAACGAATGCCGCTCCCGGACGTTTTCGCCGCAATATGCCTGATCCGATC
CCGGTGGTGGTGCTGGGTCGTCTGGCGGTAGATGAATCATTGCATGGTCAGGGCGTTGGCCGAGCGCTGGTGCGCGATGC
CGGGCTGCGGGTGATTCAGGTGGCGGATACCATCGGTATTCGCGGGATGTTGGTTCATGCGCTGTCTGATGAGGCGAGAG
CATTTTACCTACGGGTGGGGTTTGAGCCTTCACCGATTGACCCGATGATGTTGATGGTGACGTTAGGGGATTTGGTGGGG
AGTTTATCAATTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10944.55 Da
• Isoelectric Point: 10.1968

>AT23968 WP_015847873.1 NC_012912:c4024880-4024587 [Dickeya chrysanthemi Ech1591]
MPVTNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPEAYQEFLARLDQAPSPN
TALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT23968 NC_012912:c4024880-4024587 [Dickeya chrysanthemi Ech1591]
ATGCCAGTGACGAACAGCGTTTCTGTTAAACGCGAAACATTGAATTTACGCATCAAGCCCGCTGAACGCGACCTTATCGA
TCGCGCTGCGAAGGCCAGAGGAAAAAATCGTACTGATTTTGTCCTTGAGGCTGCGCGTGCCGCCGCAGAGGAGGCGTTGA
TCGATCAACGCATCATCATGGCGGATCCTGAAGCTTATCAGGAATTCCTTGCTCGCTTGGATCAGGCTCCTTCGCCGAAT
ACTGCGCTGCGCAAAACCATGCAGACGCCTGCACCGTGGGAACAGAAAAAATGA

Structures

Toxin

• AlphaFold DB: C6CKJ1

Antitoxin

• AlphaFold DB: C6CKJ2