Detailed information of TA system TA023967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4020431..4020957
• Replicon: chromosome
• Accession: NC_012912
• Organism: Dickeya zeae Ech1591

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: C6CKI8
• Locus tag: DD1591_RS17615
• Protein ID: WP_015847869.1
• Coordinates: 4020652..4020957 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6CKI7
• Locus tag: DD1591_RS17610
• Protein ID: WP_015847868.1
• Coordinates: 4020431..4020649 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DD1591_RS17585	4015690..4016265	+	576	WP_015847863.1	transcriptional regulator	-
DD1591_RS17595	4016665..4017924	+	1260	WP_015847864.1	integrase arm-type DNA-binding domain-containing protein	-
DD1591_RS21775	4018065..4018253	-	189	WP_033111580.1	hypothetical protein	-
DD1591_RS17600	4018376..4018717	+	342	WP_015847866.1	hypothetical protein	-
DD1591_RS17605	4019629..4020372	+	744	WP_015847867.1	hypothetical protein	-
DD1591_RS17610	4020431..4020649	+	219	WP_015847868.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
DD1591_RS17615	4020652..4020957	+	306	WP_015847869.1	type II toxin-antitoxin system toxin CcdB	Toxin
DD1591_RS17620	4021156..4021998	-	843	WP_015847870.1	DNA adenine methylase	-
DD1591_RS17625	4022084..4023805	-	1722	WP_015847871.1	AAA family ATPase	-
DD1591_RS17630	4024096..4024590	-	495	WP_015847872.1	GNAT family N-acetyltransferase	-
DD1591_RS17635	4024587..4024880	-	294	WP_015847873.1	DUF1778 domain-containing protein	-
DD1591_RS17640	4025405..4025905	-	501	WP_015847874.1	serine acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4016488..4025136	8648

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11674.53 Da
• Isoelectric Point: 5.1312

>T23967 WP_015847869.1 NC_012912:4020652-4020957 [Dickeya chrysanthemi Ech1591]
MQFIVYQYKRASHYKMFVDVQSNIVETPKRRMVIPLIESHHLSEKVNKTLFPQIRIDGEDYRLMTTELSSVPVEVIGGEI
ADLGEYADEIKDAINLMFLGI

Nucleotide

• Length: 306 bp

>T23967 NC_012912:4020652-4020957 [Dickeya chrysanthemi Ech1591]
ATGCAATTCATTGTTTATCAATACAAACGCGCCAGCCACTACAAAATGTTTGTCGATGTGCAAAGCAATATTGTCGAGAC
ACCCAAGCGGCGCATGGTCATCCCGCTTATCGAATCGCATCACCTGTCTGAGAAAGTGAATAAAACGCTGTTCCCTCAGA
TCCGCATCGACGGCGAAGACTATCGGCTGATGACGACTGAACTATCGAGCGTTCCCGTTGAGGTTATTGGTGGAGAAATC
GCGGATCTTGGCGAGTATGCGGATGAGATTAAGGATGCGATTAATCTTATGTTTTTGGGGATTTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8243.24 Da
• Isoelectric Point: 7.2038

>AT23967 WP_015847868.1 NC_012912:4020431-4020649 [Dickeya chrysanthemi Ech1591]
MKHRVSVTVDKDNYQVLSAAGINISGLVNDVIGKEARRIKAEEWKKENREGMEEVARFIAQHGSFADENRNW

Nucleotide

• Length: 219 bp

>AT23967 NC_012912:4020431-4020649 [Dickeya chrysanthemi Ech1591]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAATTATCAGGTTCTGAGTGCTGCCGGAATCAATATCTCTGGGCT
GGTGAATGATGTCATTGGCAAAGAAGCCCGCCGCATAAAGGCAGAGGAGTGGAAGAAAGAAAACCGCGAAGGAATGGAGG
AAGTCGCCCGATTTATCGCACAGCATGGCTCCTTTGCGGATGAAAACAGGAACTGGTGA

Structures

Toxin

• AlphaFold DB: C6CKI8

Antitoxin

• AlphaFold DB: C6CKI7