Detailed information of TA system TA239667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 66010..66653
• Replicon: plasmid unnamed2
• Accession: NZ_CP094325
• Organism: Edwardsiella anguillarum strain Eco2413

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: MQ084_RS20155
• Protein ID: WP_283292435.1
• Coordinates: 66237..66653 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6C0NE67
• Locus tag: MQ084_RS20150
• Protein ID: WP_008322219.1
• Coordinates: 66010..66240 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQ084_RS20135 (MQ084_20115)	62115..63203	+	1089	WP_283292455.1	transcriptional repressor PifC	-
MQ084_RS20140 (MQ084_20120)	63205..64074	+	870	WP_068872224.1	hypothetical protein	-
MQ084_RS20145 (MQ084_20125)	64123..65694	-	1572	WP_283292432.1	AAA family ATPase	-
MQ084_RS20150 (MQ084_20130)	66010..66240	+	231	WP_008322219.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MQ084_RS20155 (MQ084_20135)	66237..66653	+	417	WP_283292435.1	type II toxin-antitoxin system VapC family toxin	Toxin
MQ084_RS20160 (MQ084_20140)	66727..68289	+	1563	WP_283292438.1	AAA family ATPase	-
MQ084_RS20165 (MQ084_20145)	68274..69296	+	1023	WP_283292440.1	DNA helicase UvrD	-
MQ084_RS20170 (MQ084_20150)	69457..70176	-	720	Protein_73	IS3 family transposase	-
MQ084_RS20175 (MQ084_20155)	70233..71033	-	801	WP_283292457.1	IS21-like element helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	papD / papC	1..91494	91494

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15132.48 Da
• Isoelectric Point: 7.3236

>T239667 WP_283292435.1 NZ_CP094325:66237-66653 [Edwardsiella anguillarum]
VNKTYMLDTNICSFIMREQPDVVLKHLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKLSLRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T239667 NZ_LS483301:c1608467-1608222 [Staphylococcus aureus]
ATGAACATTCAAGAAGCAACTAAGATAGCTACAAAAAATCTTGTCTCTATGACACGGAAAGATTGGAAAGAAAGTCATCG
AACTAAGATATTACCAACAAATGATAGTTTTTTACAATGCATCATTTCAAATAGCGATGGGACAAACCTTATCAGATATT
GGCAACCTTCAGCCGATGACCTCATGGCAAATGATTGGGAAGTTATAAACCCAACTAGAGACCAGGAATTATTGAAGCAA
TTTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8736.84 Da
• Isoelectric Point: 4.4692

>AT239667 WP_008322219.1 NZ_CP094325:66010-66240 [Edwardsiella anguillarum]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFNL

Nucleotide

• Length: 231 bp

>AT239667 NZ_LS483301:1607888-1608253 [Staphylococcus aureus]
ATGCCCTTGATATCTGATGAATTTGATACACTTACTAAAGACCAACAATATATCTTGTCCGTACTCTACAAAGATTATTT
AGAATGTGTAAAGTTAGGTTCGGTTAAATTAACCTGCAATAATTTTGGAAGTGCTAAAGATATACATACAAAGTATTTTC
AAAAACTACATTTCGAAGATGTAAAATACGATTTAAATAAACTTAAAAACTCTGGGTTCCTAAACGGCGTGTATGCTAGT
AACACTATTTATCATGTAACAATTTCAGACAAGACTGTTGTTTACTTTGAAAATGAGTTTAAAAACAATTTAAAAAGTAT
CATTGATAGCATTTCTAAAATTGCTTCAATAATTCCTGGTCTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A6C0NE67