Detailed information of TA system TA239593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 20051..20694
• Replicon: plasmid unnamed1
• Accession: NZ_CP094318
• Organism: Edwardsiella anguillarum strain R17-63

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: MQ083_RS19065
• Protein ID: WP_283292435.1
• Coordinates: 20278..20694 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6C0NE67
• Locus tag: MQ083_RS19060
• Protein ID: WP_008322219.1
• Coordinates: 20051..20281 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQ083_RS19045 (MQ083_19010)	16156..17244	+	1089	WP_283292455.1	transcriptional repressor PifC	-
MQ083_RS19050 (MQ083_19015)	17246..18115	+	870	WP_068872224.1	hypothetical protein	-
MQ083_RS19055 (MQ083_19020)	18164..19735	-	1572	WP_283292432.1	AAA family ATPase	-
MQ083_RS19060 (MQ083_19025)	20051..20281	+	231	WP_008322219.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MQ083_RS19065 (MQ083_19030)	20278..20694	+	417	WP_283292435.1	type II toxin-antitoxin system VapC family toxin	Toxin
MQ083_RS19070 (MQ083_19035)	20768..22330	+	1563	WP_283292438.1	AAA family ATPase	-
MQ083_RS19075 (MQ083_19040)	22315..23337	+	1023	WP_283292440.1	DNA helicase UvrD	-
MQ083_RS19080 (MQ083_19045)	23498..24217	-	720	Protein_24	IS3 family transposase	-
MQ083_RS19085 (MQ083_19050)	24274..25074	-	801	WP_283292457.1	IS21-like element helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	papD / papC	1..91492	91492

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15132.48 Da
• Isoelectric Point: 7.3236

>T239593 WP_283292435.1 NZ_CP094318:20278-20694 [Edwardsiella anguillarum]
VNKTYMLDTNICSFIMREQPDVVLKHLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKLSLRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T239593 NZ_LS399318:2816616-2816963 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8736.84 Da
• Isoelectric Point: 4.4692

>AT239593 WP_008322219.1 NZ_CP094318:20051-20281 [Edwardsiella anguillarum]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFNL

Nucleotide

• Length: 231 bp

>AT239593 NZ_LS399318:2816963-2817256 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAAGTCTCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6C0NE67