Detailed information of TA system TA239566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5577..6202
• Replicon: plasmid unnamed6
• Accession: NZ_CP094314
• Organism: Edwardsiella anguillarum strain R18-14-16

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MQ086_RS19540
• Protein ID: WP_098142458.1
• Coordinates: 5577..5975 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MQ086_RS19545
• Protein ID: WP_098142459.1
• Coordinates: 5975..6202 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQ086_RS19530 (MQ086_19505)	2814..3209	+	396	WP_283292985.1	hypothetical protein	-
MQ086_RS19535 (MQ086_19510)	3296..5499	+	2204	Protein_2	type IV conjugative transfer system coupling protein TraD	-
MQ086_RS19540 (MQ086_19515)	5577..5975	-	399	WP_098142458.1	tRNA(fMet)-specific endonuclease VapC	Toxin
MQ086_RS19545 (MQ086_19520)	5975..6202	-	228	WP_098142459.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	papC / papD	1..91497	91497

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14835.08 Da
• Isoelectric Point: 7.9270

>T239566 WP_098142458.1 NZ_CP094314:c5975-5577 [Edwardsiella anguillarum]
MLKYMLDTNICIFTLKNRPAQVRDKFNLHHNQMCISSITLMELIYGAEKSQSPEHNLAVIEGFIARLTVLNYDAPAAAHS
GQLRAEQARLGQPIGPYDQMIAGHARSQGLILVTNNTREFARVSGLRIEDWV

Nucleotide

• Length: 399 bp

>T239566 NZ_LR898876:39174-39335 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACACCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8384.40 Da
• Isoelectric Point: 4.7405

>AT239566 WP_098142459.1 NZ_CP094314:c6202-5975 [Edwardsiella anguillarum]
METTVFLSNRSQAVRLPKAVALPSDVKRVDIVAVGRTRIITPAGESWDSWFEGEGVSTDFMASREQPKGQEREDF

Nucleotide

• Length: 228 bp

>AT239566 NZ_LR898876:c39121-38917 [Escherichia coli]
AGACATACAGGGGGCCTCGTGGGTTAATGAAAAATTAACTACGGGGCTTTCTGCCTTCTGCCACACAACACGGTAACAAA
CCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCGCACTGACCACACTCAC
TTTCCCTGAAAATAATCCGGTCGTTCAGCCAGTTCACGGGCAATC