Detailed information of TA system TA239553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 12681..13324
• Replicon: plasmid unnamed5
• Accession: NZ_CP094312
• Organism: Edwardsiella anguillarum strain R18-14-2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: MQ082_RS19860
• Protein ID: WP_283292435.1
• Coordinates: 12681..13097 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6C0NE67
• Locus tag: MQ082_RS19865
• Protein ID: WP_008322219.1
• Coordinates: 13094..13324 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQ082_RS19840 (MQ082_19815)	8301..9101	+	801	WP_283292457.1	IS21-like element helper ATPase IstB	-
MQ082_RS19845 (MQ082_19820)	9158..9877	+	720	Protein_9	IS3 family transposase	-
MQ082_RS19850 (MQ082_19825)	10038..11060	-	1023	WP_283292440.1	DNA helicase UvrD	-
MQ082_RS19855 (MQ082_19830)	11045..12607	-	1563	WP_283292438.1	AAA family ATPase	-
MQ082_RS19860 (MQ082_19835)	12681..13097	-	417	WP_283292435.1	type II toxin-antitoxin system VapC family toxin	Toxin
MQ082_RS19865 (MQ082_19840)	13094..13324	-	231	WP_008322219.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MQ082_RS19870 (MQ082_19845)	13640..15211	+	1572	WP_283292432.1	AAA family ATPase	-
MQ082_RS19875 (MQ082_19850)	15260..16129	-	870	WP_068872224.1	hypothetical protein	-
MQ082_RS19880 (MQ082_19855)	16131..17219	-	1089	WP_283292455.1	transcriptional repressor PifC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	papC / papD	1..91490	91490

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15132.48 Da
• Isoelectric Point: 7.3236

>T239553 WP_283292435.1 NZ_CP094312:c13097-12681 [Edwardsiella anguillarum]
VNKTYMLDTNICSFIMREQPDVVLKHLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKLSLRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T239553 NZ_LR898874:3448087-3448473 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8736.84 Da
• Isoelectric Point: 4.4692

>AT239553 WP_008322219.1 NZ_CP094312:c13324-13094 [Edwardsiella anguillarum]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFNL

Nucleotide

• Length: 231 bp

>AT239553 NZ_LR898874:3448463-3448792 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A6C0NE67