Detailed information of TA system TA239541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 56971..57614
• Replicon: plasmid unnamed4
• Accession: NZ_CP094310
• Organism: Edwardsiella anguillarum strain R18-14-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: MQ096_RS20580
• Protein ID: WP_283292435.1
• Coordinates: 57198..57614 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6C0NE67
• Locus tag: MQ096_RS20575
• Protein ID: WP_008322219.1
• Coordinates: 56971..57201 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQ096_RS20555 (MQ096_20540)	53077..54165	+	1089	WP_283292455.1	transcriptional repressor PifC	-
MQ096_RS20560 (MQ096_20545)	54167..55036	+	870	WP_068872224.1	hypothetical protein	-
MQ096_RS20565 (MQ096_20550)	55085..55735	-	651	WP_283318049.1	hypothetical protein	-
MQ096_RS20570 (MQ096_20555)	55708..56655	-	948	WP_283318050.1	AAA family ATPase	-
MQ096_RS20575 (MQ096_20560)	56971..57201	+	231	WP_008322219.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MQ096_RS20580 (MQ096_20565)	57198..57614	+	417	WP_283292435.1	type II toxin-antitoxin system VapC family toxin	Toxin
MQ096_RS20585 (MQ096_20570)	57688..59250	+	1563	WP_283292438.1	AAA family ATPase	-
MQ096_RS20590 (MQ096_20575)	59235..60257	+	1023	WP_283292440.1	DNA helicase UvrD	-
MQ096_RS20595 (MQ096_20580)	60418..61137	-	720	Protein_61	IS3 family transposase	-
MQ096_RS20600 (MQ096_20585)	61194..61994	-	801	WP_283292457.1	IS21-like element helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	papD / papC / papC	1..81718	81718

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15132.48 Da
• Isoelectric Point: 7.3236

>T239541 WP_283292435.1 NZ_CP094310:57198-57614 [Edwardsiella anguillarum]
VNKTYMLDTNICSFIMREQPDVVLKHLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKLSLRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T239541 NZ_LR898874:1022897-1023304 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8736.84 Da
• Isoelectric Point: 4.4692

>AT239541 WP_008322219.1 NZ_CP094310:56971-57201 [Edwardsiella anguillarum]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFNL

Nucleotide

• Length: 231 bp

>AT239541 NZ_LR898874:1022650-1022916 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6C0NE67