Detailed information of TA system TA239540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 13047..13672
• Replicon: plasmid unnamed4
• Accession: NZ_CP094310
• Organism: Edwardsiella anguillarum strain R18-14-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MQ096_RS20340
• Protein ID: WP_098142458.1
• Coordinates: 13274..13672 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MQ096_RS20335
• Protein ID: WP_098142459.1
• Coordinates: 13047..13274 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQ096_RS20335 (MQ096_20320)	13047..13274	+	228	WP_098142459.1	toxin-antitoxin system antitoxin VapB	Antitoxin
MQ096_RS20340 (MQ096_20325)	13274..13672	+	399	WP_098142458.1	tRNA(fMet)-specific endonuclease VapC	Toxin
MQ096_RS20345 (MQ096_20330)	13750..15954	-	2205	WP_283292376.1	type IV conjugative transfer system coupling protein TraD	-
MQ096_RS20350 (MQ096_20335)	16041..16538	-	498	WP_283292378.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	papD / papC / papC	1..81718	81718

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14835.08 Da
• Isoelectric Point: 7.9270

>T239540 WP_098142458.1 NZ_CP094310:13274-13672 [Edwardsiella anguillarum]
MLKYMLDTNICIFTLKNRPAQVRDKFNLHHNQMCISSITLMELIYGAEKSQSPEHNLAVIEGFIARLTVLNYDAPAAAHS
GQLRAEQARLGQPIGPYDQMIAGHARSQGLILVTNNTREFARVSGLRIEDWV

Nucleotide

• Length: 399 bp

>T239540 NZ_LR898874:c853459-853082 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8384.40 Da
• Isoelectric Point: 4.7405

>AT239540 WP_098142459.1 NZ_CP094310:13047-13274 [Edwardsiella anguillarum]
METTVFLSNRSQAVRLPKAVALPSDVKRVDIVAVGRTRIITPAGESWDSWFEGEGVSTDFMASREQPKGQEREDF

Nucleotide

• Length: 228 bp

>AT239540 NZ_LR898874:c853916-853536 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAACAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG