Detailed information of TA system TA239539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 37511..38154
• Replicon: plasmid unnamed8
• Accession: NZ_CP094309
• Organism: Edwardsiella anguillarum strain R18-14-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: MQ096_RS20020
• Protein ID: WP_283292435.1
• Coordinates: 37738..38154 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6C0NE67
• Locus tag: MQ096_RS20015
• Protein ID: WP_008322219.1
• Coordinates: 37511..37741 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQ096_RS19995 (MQ096_19980)	33615..34703	+	1089	WP_283292455.1	transcriptional repressor PifC	-
MQ096_RS20000 (MQ096_19985)	34705..35574	+	870	WP_068872224.1	hypothetical protein	-
MQ096_RS20005 (MQ096_19990)	35623..37038	-	1416	WP_283318048.1	AAA family ATPase	-
MQ096_RS20010 (MQ096_19995)	36953..37195	-	243	WP_283318047.1	AAA family ATPase	-
MQ096_RS20015 (MQ096_20000)	37511..37741	+	231	WP_008322219.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MQ096_RS20020 (MQ096_20005)	37738..38154	+	417	WP_283292435.1	type II toxin-antitoxin system VapC family toxin	Toxin
MQ096_RS20025 (MQ096_20010)	38228..39790	+	1563	WP_283292438.1	AAA family ATPase	-
MQ096_RS20030 (MQ096_20015)	39775..40796	+	1022	Protein_45	DNA helicase UvrD	-
MQ096_RS20035 (MQ096_20020)	40957..41676	-	720	Protein_46	IS3 family transposase	-
MQ096_RS20040 (MQ096_20025)	41733..42533	-	801	WP_283292457.1	IS21-like element helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	papD / papC	1..91487	91487

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15132.48 Da
• Isoelectric Point: 7.3236

>T239539 WP_283292435.1 NZ_CP094309:37738-38154 [Edwardsiella anguillarum]
VNKTYMLDTNICSFIMREQPDVVLKHLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKLSLRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T239539 NZ_LR898874:c644937-644473 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTTGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8736.84 Da
• Isoelectric Point: 4.4692

>AT239539 WP_008322219.1 NZ_CP094309:37511-37741 [Edwardsiella anguillarum]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFNL

Nucleotide

• Length: 231 bp

>AT239539 NZ_LR898874:c645272-644937 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAATAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCAGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A6C0NE67