Detailed information of TA system TA023953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4441736..4442550
• Replicon: chromosome
• Accession: NC_012892
• Organism: Escherichia coli BL21(DE3)

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: B21_RS21990
• Protein ID: WP_001054376.1
• Coordinates: 4442293..4442550 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: B21_RS21985
• Protein ID: WP_001309181.1
• Coordinates: 4441736..4442281 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B21_RS21965	4437427..4438740	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
B21_RS21970	4438752..4439030	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
B21_RS21975	4439027..4440148	-	1122	WP_000010829.1	M42 family metallopeptidase	-
B21_RS25620	4440393..4440509	-	117	Protein_4163	VOC family protein	-
B21_RS25050	4440547..4440765	-	219	Protein_4164	hypothetical protein	-
B21_RS21980	4440934..4441680	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
B21_RS21985	4441736..4442281	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
B21_RS21990	4442293..4442550	-	258	WP_001054376.1	hypothetical protein	Toxin
B21_RS25475	4442927..4443172	-	246	Protein_4168	GNAT family N-acetyltransferase	-
B21_RS22005	4443288..4444528	+	1241	Protein_4169	helicase YjhR	-
B21_RS22010	4445111..4446091	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
B21_RS22015	4446156..4447262	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimB / fimE	4430155..4451906	21751

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T23953 WP_001054376.1 NC_012892:c4442550-4442293 [Escherichia coli BL21(DE3)]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T23953 NC_012892:c4442550-4442293 [Escherichia coli BL21(DE3)]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT23953 WP_001309181.1 NC_012892:c4442281-4441736 [Escherichia coli BL21(DE3)]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT23953 NC_012892:c4442281-4441736 [Escherichia coli BL21(DE3)]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8