Detailed information of TA system TA239514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 76433..77076
• Replicon: plasmid unnamed4
• Accession: NZ_CP094305
• Organism: Edwardsiella anguillarum strain R18-08

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: MQ088_RS20225
• Protein ID: WP_283292435.1
• Coordinates: 76433..76849 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6C0NE67
• Locus tag: MQ088_RS20230
• Protein ID: WP_008322219.1
• Coordinates: 76846..77076 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQ088_RS20205 (MQ088_20180)	72053..72853	+	801	WP_283292457.1	IS21-like element helper ATPase IstB	-
MQ088_RS20210 (MQ088_20185)	72910..73629	+	720	Protein_80	IS3 family transposase	-
MQ088_RS20215 (MQ088_20190)	73790..74812	-	1023	WP_283292440.1	DNA helicase UvrD	-
MQ088_RS20220 (MQ088_20195)	74797..76359	-	1563	WP_283292438.1	AAA family ATPase	-
MQ088_RS20225 (MQ088_20200)	76433..76849	-	417	WP_283292435.1	type II toxin-antitoxin system VapC family toxin	Toxin
MQ088_RS20230 (MQ088_20205)	76846..77076	-	231	WP_008322219.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MQ088_RS20235 (MQ088_20210)	77392..78963	+	1572	WP_283292432.1	AAA family ATPase	-
MQ088_RS20240 (MQ088_20215)	79012..79881	-	870	WP_068872224.1	hypothetical protein	-
MQ088_RS20245 (MQ088_20220)	79883..80971	-	1089	WP_283292455.1	transcriptional repressor PifC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	papC / papD	1..91496	91496

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15132.48 Da
• Isoelectric Point: 7.3236

>T239514 WP_283292435.1 NZ_CP094305:c76849-76433 [Edwardsiella anguillarum]
VNKTYMLDTNICSFIMREQPDVVLKHLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKLSLRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T239514 NZ_LR898868:c602000-601554 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8736.84 Da
• Isoelectric Point: 4.4692

>AT239514 WP_008322219.1 NZ_CP094305:c77076-76846 [Edwardsiella anguillarum]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFNL

Nucleotide

• Length: 231 bp

>AT239514 NZ_LR898868:c602335-602000 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A6C0NE67