Detailed information of TA system TA239501

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 78381..79006
• Replicon: plasmid unnamed7
• Accession: NZ_CP094303
• Organism: Edwardsiella anguillarum strain R18-35-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MQ095_RS20240
• Protein ID: WP_098142458.1
• Coordinates: 78608..79006 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MQ095_RS20235
• Protein ID: WP_098142459.1
• Coordinates: 78381..78608 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQ095_RS20235 (MQ095_20210)	78381..78608	+	228	WP_098142459.1	toxin-antitoxin system antitoxin VapB	Antitoxin
MQ095_RS20240 (MQ095_20215)	78608..79006	+	399	WP_098142458.1	tRNA(fMet)-specific endonuclease VapC	Toxin
MQ095_RS20245 (MQ095_20220)	79084..81288	-	2205	WP_283292376.1	type IV conjugative transfer system coupling protein TraD	-
MQ095_RS20250 (MQ095_20225)	81375..81872	-	498	WP_283292378.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	papD / papC	1..91492	91492

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14835.08 Da
• Isoelectric Point: 7.9270

>T239501 WP_098142458.1 NZ_CP094303:78608-79006 [Edwardsiella anguillarum]
MLKYMLDTNICIFTLKNRPAQVRDKFNLHHNQMCISSITLMELIYGAEKSQSPEHNLAVIEGFIARLTVLNYDAPAAAHS
GQLRAEQARLGQPIGPYDQMIAGHARSQGLILVTNNTREFARVSGLRIEDWV

Nucleotide

• Length: 399 bp

>T239501 NZ_LR890751:c4747436-4747152 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8384.40 Da
• Isoelectric Point: 4.7405

>AT239501 WP_098142459.1 NZ_CP094303:78381-78608 [Edwardsiella anguillarum]
METTVFLSNRSQAVRLPKAVALPSDVKRVDIVAVGRTRIITPAGESWDSWFEGEGVSTDFMASREQPKGQEREDF

Nucleotide

• Length: 228 bp

>AT239501 NZ_LR890751:c4747668-4747426 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA