Detailed information of TA system TA239474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3081491..3082408
• Replicon: chromosome
• Accession: NZ_CP094294
• Organism: Bacillus velezensis strain AP3

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: MRS49_RS15980
• Protein ID: WP_007407256.1
• Coordinates: 3081662..3082408 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: MRS49_RS15975
• Protein ID: WP_003154807.1
• Coordinates: 3081491..3081661 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRS49_RS15935	3076724..3078346	+	1623	WP_032874601.1	pyocin knob domain-containing protein	-
MRS49_RS15940	3078359..3078730	+	372	WP_032874603.1	XkdW family protein	-
MRS49_RS15945	3078735..3078932	+	198	WP_032874605.1	XkdX family protein	-
MRS49_RS15950	3078989..3079750	+	762	WP_032874607.1	hypothetical protein	-
MRS49_RS15955	3079802..3080065	+	264	WP_003154815.1	hemolysin XhlA family protein	-
MRS49_RS15960	3080079..3080342	+	264	WP_003154813.1	phage holin	-
MRS49_RS15965	3080356..3081234	+	879	WP_032874609.1	N-acetylmuramoyl-L-alanine amidase	-
MRS49_RS15970	3081269..3081394	-	126	WP_003154809.1	hypothetical protein	-
MRS49_RS15975	3081491..3081661	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
MRS49_RS15980	3081662..3082408	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
MRS49_RS15985	3082513..3083511	-	999	WP_032874611.1	inorganic phosphate transporter	-
MRS49_RS15990	3083524..3084141	-	618	WP_032874614.1	DUF47 domain-containing protein	-
MRS49_RS15995	3084427..3085743	-	1317	WP_032874616.1	amino acid permease	-
MRS49_RS16000	3086065..3087015	+	951	WP_032874618.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3037737..3140260	102523

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T239474 WP_007407256.1 NZ_CP094294:c3082408-3081662 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T239474 NZ_LR890726:3948931-3949149 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT239474 WP_003154807.1 NZ_CP094294:c3081661-3081491 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT239474 NZ_LR890726:3948530-3948904 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14