Detailed information of TA system TA239473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2330252..2330889
• Replicon: chromosome
• Accession: NZ_CP094294
• Organism: Bacillus velezensis strain AP3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: MRS49_RS11995
• Protein ID: WP_003156187.1
• Coordinates: 2330539..2330889 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: MRS49_RS11990
• Protein ID: WP_003156188.1
• Coordinates: 2330252..2330533 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRS49_RS11970	2326617..2327216	-	600	WP_032872997.1	rhomboid family intramembrane serine protease	-
MRS49_RS11975	2327309..2327674	+	366	WP_007609580.1	holo-ACP synthase	-
MRS49_RS11980	2327839..2328846	+	1008	WP_032872999.1	outer membrane lipoprotein carrier protein LolA	-
MRS49_RS11985	2328963..2330132	+	1170	WP_032873001.1	alanine racemase	-
MRS49_RS11990	2330252..2330533	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
MRS49_RS11995	2330539..2330889	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
MRS49_RS12000	2331007..2331828	+	822	WP_003156182.1	STAS domain-containing protein	-
MRS49_RS12005	2331833..2332198	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
MRS49_RS12010	2332201..2332602	+	402	WP_003156178.1	anti-sigma regulatory factor	-
MRS49_RS12015	2332614..2333621	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
MRS49_RS12020	2333685..2334014	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
MRS49_RS12025	2334011..2334493	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
MRS49_RS12030	2334459..2335247	+	789	WP_032873003.1	RNA polymerase sigma factor SigB	-
MRS49_RS12035	2335247..2335849	+	603	WP_007410234.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T239473 WP_003156187.1 NZ_CP094294:2330539-2330889 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T239473 NZ_LR890726:c3130010-3129834 [Klebsiella pneumoniae]
GTGAAGCAAAGCGAGTTGAGGCGTTGGCTTGCAGCTCAAGGGGCAGAATTTAAAGATGGTACTAACCACTTGAAAATCTA
TCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTTAAGC
AACTCGGCATCAAATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT239473 WP_003156188.1 NZ_CP094294:2330252-2330533 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT239473 NZ_LR890726:c3129786-3129379 [Klebsiella pneumoniae]
ATGCGATACCCGGTAATTTTTGAGCATGACGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACGGG
AGGAGAGACCAGGGAGGAAGCGTTAGAAATGGCGCAAGATGCCCTGGTGACGGCGTTTGATTTTTACTTTGACGACCGCC
GGGAGATTCCCGCTCCATCAGCAGAAGGTGATGCGTTTGTTGAGGTTCCGGCCAGCGTGGCAGCTAAAGTATTGCTGTTA
AACCGTCTGGTCAGCACCAACACCAGCAATGCTGATCTCGCCCGCATGATTAACACGCGCCCACAGGAAGTGCAGCGCAT
CGTGTCTCTTGGTCACAGCACCAAAATCGATACGATCCAAAAAGCGCTATCAGCGTTGGGGCAAAAGATGGAAATTGTCG
TCCACTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5