Detailed information of TA system TA239463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 162078..162600
• Replicon: plasmid pNY1464-1
• Accession: NZ_CP094281
• Organism: Klebsiella aerogenes strain NY1464

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A7H0EVH6
• Locus tag: MQE09_RS25380
• Protein ID: WP_014839901.1
• Coordinates: 162078..162362 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A7H0EVH5
• Locus tag: MQE09_RS25385
• Protein ID: WP_014839900.1
• Coordinates: 162352..162600 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQE09_RS25340 (MQE09_25340)	157334..157543	+	210	WP_125316032.1	hypothetical protein	-
MQE09_RS25345 (MQE09_25345)	157606..158550	+	945	WP_014839903.1	DsbA family protein	-
MQE09_RS25350 (MQE09_25350)	158631..159152	+	522	WP_048336588.1	hypothetical protein	-
MQE09_RS25355 (MQE09_25355)	159149..159751	+	603	WP_014839902.1	HAD domain-containing protein	-
MQE09_RS26360	159992..160711	+	720	WP_042934502.1	cation:proton antiporter	-
MQE09_RS25370 (MQE09_25370)	160728..161405	-	678	WP_042934501.1	hypothetical protein	-
MQE09_RS25375 (MQE09_25375)	161411..161887	-	477	WP_042934526.1	hypothetical protein	-
MQE09_RS25380 (MQE09_25380)	162078..162362	-	285	WP_014839901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MQE09_RS25385 (MQE09_25385)	162352..162600	-	249	WP_014839900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MQE09_RS25390 (MQE09_25390)	162941..164344	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
MQE09_RS25395 (MQE09_25395)	164373..165005	-	633	WP_001567369.1	hypothetical protein	-
MQE09_RS25400 (MQE09_25400)	165549..166157	-	609	WP_024266306.1	DUF2913 family protein	-
MQE09_RS25405 (MQE09_25405)	166294..166692	-	399	WP_014839899.1	H-NS family nucleoid-associated regulatory protein	-
MQE09_RS25410 (MQE09_25410)	167066..167422	-	357	WP_017901071.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / qacE / blaIMP-26 / aac(3)-IId / blaTEM-1B / mph(A) / blaDHA-1 / qnrB4 / aadA16 / dfrA27 / ARR-3	-	1..326718	326718
	-	flank	IS/Tn	-	-	162941..164344	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10866.46 Da
• Isoelectric Point: 10.5831

>T239463 WP_014839901.1 NZ_CP094281:c162362-162078 [Klebsiella aerogenes]
MTYKLAFNESALKEWKKLGHTIREQFKKKLSERLQNPRVPAAQLHGGKDQYKIKLHGAGYRLVYSVNDEIVTVTVIGVGK
RNNDDIYNATRHRN

Nucleotide

• Length: 285 bp

>T239463 NZ_LR890722:c4675800-4675516 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9071.29 Da
• Isoelectric Point: 3.8832

>AT239463 WP_014839900.1 NZ_CP094281:c162600-162352 [Klebsiella aerogenes]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEMLEDQELGRIADERIGEDRIEVNID
DL

Nucleotide

• Length: 249 bp

>AT239463 NZ_LR890722:c4676032-4675790 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A7H0EVH6

Antitoxin

• AlphaFold DB: A0A7H0EVH5