Detailed information of TA system TA239459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2588295..2589034
• Replicon: chromosome
• Accession: NZ_CP094280
• Organism: Klebsiella aerogenes strain NY1464

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: MQE09_RS12450
• Protein ID: WP_224108231.1
• Coordinates: 2588549..2589034 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A0H3FSM9
• Locus tag: MQE09_RS12445
• Protein ID: WP_015705366.1
• Coordinates: 2588295..2588561 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQE09_RS12425 (2584577)	2584577..2585194	+	618	WP_224108234.1	response regulator	-
MQE09_RS12430 (2585290)	2585290..2585787	+	498	WP_224108233.1	heme-binding protein	-
MQE09_RS12435 (2586202)	2586202..2586732	+	531	WP_047041122.1	N-acetyltransferase	-
MQE09_RS12440 (2586787)	2586787..2588022	-	1236	WP_224108232.1	MFS transporter	-
MQE09_RS12445 (2588295)	2588295..2588561	+	267	WP_015705366.1	DUF1778 domain-containing protein	Antitoxin
MQE09_RS12450 (2588549)	2588549..2589034	+	486	WP_224108231.1	GNAT family N-acetyltransferase	Toxin
MQE09_RS12455 (2589106)	2589106..2590722	+	1617	WP_182014897.1	FAD-NAD(P)-binding protein	-
MQE09_RS12460 (2590841)	2590841..2591941	+	1101	WP_224108230.1	NADH:flavin oxidoreductase/NADH oxidase	-
MQE09_RS12465 (2591998)	2591998..2592156	-	159	WP_002903230.1	YqaE/Pmp3 family membrane protein	-
MQE09_RS12470 (2592417)	2592417..2593487	+	1071	WP_108418538.1	mannonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17627.34 Da
• Isoelectric Point: 8.8574

>T239459 WP_224108231.1 NZ_CP094280:2588549-2589034 [Klebsiella aerogenes]
MGKISAPAPLSPHHQIAEFCCGETVLDQWLKQRGMKNQAQGAARTFVVCKEDSHQVVGFYSLATGSVNHTEATGGLRRNM
PDPIPVIILARLAIDCAFHGQGLGADLLHDALLRSYRVAENVGVRALMVHALTDSAKRFYLYHGFKASTTQERTLFLALP
K

Nucleotide

• Length: 486 bp

>T239459 NZ_LR890722:2372498-2372815 [Klebsiella pneumoniae]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10085.56 Da
• Isoelectric Point: 4.6558

>AT239459 WP_015705366.1 NZ_CP094280:2588295-2588561 [Klebsiella aerogenes]
MKSDIQLNIRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNLSDEQYAEFIDMLDAPVEANPALDKLL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT239459 NZ_LR890722:2372891-2373187 [Klebsiella pneumoniae]
ATGAAAGACGAATTGTTTGCCGATCTGCTGGCGAGTGCAGAAGAAATGGTCAGGATCGAGAAAGGTGAACAAATGCCGAT
GGAGCAGCACGTTCATACCTTCAGCGAAATTGACGTGAAAGCGATACGTGAAGCCACAGGTTTAAAACAGCAGGACTTTG
CAGCGGCCGTTGGCGTTAGCTACGAGTTGGTTAAAAGCTGGGAAACGAAACGACGTCAGCCAACCGGTGCAGCCAGAAAA
CTGCTTTTATTGCTTCAGGGTAATCCATTGATTATCAACTTGTTGAAGGTTGTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3FSM9