Detailed information of TA system TA239447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 198705..199227
• Replicon: plasmid pNY1688-4
• Accession: NZ_CP094276
• Organism: Klebsiella aerogenes strain NY1688

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A2J4R0S6
• Locus tag: MQE04_RS28615
• Protein ID: WP_004181778.1
• Coordinates: 198943..199227 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: MQE04_RS28610
• Protein ID: WP_004181777.1
• Coordinates: 198705..198953 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQE04_RS28590 (MQE04_28590)	193914..194735	-	822	WP_004181772.1	hypothetical protein	-
MQE04_RS28595 (MQE04_28595)	194797..195150	-	354	WP_004181774.1	hypothetical protein	-
MQE04_RS28600 (MQE04_28600)	195295..196281	-	987	WP_025368599.1	hypothetical protein	-
MQE04_RS28605 (MQE04_28605)	196615..198414	-	1800	WP_004181776.1	ATP-dependent helicase	-
MQE04_RS28610 (MQE04_28610)	198705..198953	+	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MQE04_RS28615 (MQE04_28615)	198943..199227	+	285	WP_004181778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MQE04_RS28620 (MQE04_28620)	199244..199345	-	102	Protein_212	IS200/IS605 family transposase	-
MQE04_RS28625 (MQE04_28625)	199381..200607	+	1227	WP_040209639.1	RNA-guided endonuclease TnpB family protein	-
MQE04_RS28630 (MQE04_28630)	200878..201102	-	225	Protein_214	transposase	-
MQE04_RS28635 (MQE04_28635)	201181..201609	-	429	WP_077257669.1	IS200/IS605 family transposase	-
MQE04_RS28640 (MQE04_28640)	201645..202832	+	1188	WP_040209642.1	RNA-guided endonuclease TnpB family protein	-
MQE04_RS28645 (MQE04_28645)	202877..203248	-	372	WP_040209644.1	hypothetical protein	-
MQE04_RS28650 (MQE04_28650)	203245..203589	-	345	WP_223811496.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E) / armA / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr	-	1..239700	239700

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10970.69 Da
• Isoelectric Point: 10.6516

>T239447 WP_004181778.1 NZ_CP094276:198943-199227 [Klebsiella aerogenes]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Nucleotide

• Length: 285 bp

>T239447 NZ_LR890719:3895574-3895792 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT239447 WP_004181777.1 NZ_CP094276:198705-198953 [Klebsiella aerogenes]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT239447 NZ_LR890719:3895173-3895547 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A2J4R0S6

Antitoxin

• AlphaFold DB: A0A2J4R0U8