Detailed information of TA system TA239444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 43475..43955
• Replicon: plasmid pNY1688-3
• Accession: NZ_CP094275
• Organism: Klebsiella aerogenes strain NY1688

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A223LMB9
• Locus tag: MQE04_RS27530
• Protein ID: WP_058998978.1
• Coordinates: 43698..43955 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: A0A223LLF6
• Locus tag: MQE04_RS27525
• Protein ID: WP_072223385.1
• Coordinates: 43475..43708 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQE04_RS27490 (MQE04_27490)	39284..39691	+	408	WP_072223386.1	helix-turn-helix transcriptional regulator	-
MQE04_RS27495 (MQE04_27495)	39688..40857	-	1170	WP_058998988.1	MobP1 family relaxase	-
MQE04_RS27500 (MQE04_27500)	40861..41406	-	546	WP_058998986.1	hypothetical protein	-
MQE04_RS27505 (MQE04_27505)	41752..42036	-	285	WP_058998984.1	putative addiction module antidote protein	-
MQE04_RS27510 (MQE04_27510)	42038..42334	-	297	WP_013583078.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MQE04_RS27515 (MQE04_27515)	42673..42810	-	138	WP_161400388.1	hypothetical protein	-
MQE04_RS27520 (MQE04_27520)	42857..43069	-	213	WP_058998981.1	hypothetical protein	-
MQE04_RS27525 (MQE04_27525)	43475..43708	-	234	WP_072223385.1	hypothetical protein	Antitoxin
MQE04_RS27530 (MQE04_27530)	43698..43955	-	258	WP_058998978.1	hypothetical protein	Toxin
MQE04_RS27535 (MQE04_27535)	43952..44494	-	543	WP_058998977.1	hypothetical protein	-
MQE04_RS27540 (MQE04_27540)	44494..44700	-	207	WP_058998975.1	hypothetical protein	-
MQE04_RS27545 (MQE04_27545)	44712..44927	-	216	WP_058998973.1	hypothetical protein	-
MQE04_RS27550 (MQE04_27550)	44969..45490	-	522	WP_058998971.1	J domain-containing protein	-
MQE04_RS27555 (MQE04_27555)	45603..45824	+	222	WP_058998969.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2	-	1..46193	46193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (7-36)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9777.10 Da
• Isoelectric Point: 9.3899

>T239444 WP_058998978.1 NZ_CP094275:c43955-43698 [Klebsiella aerogenes]
MKKSKGDAQYYLEKEGDIYHLVKRVKTFSKKLTQGKTKATTKTVSDFSFTKNNFEDIDFNANGLREKDKSIIVQMVEEIE
GLHAD

Nucleotide

• Length: 258 bp

>T239444 NZ_LR890719:827310-827720 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8887.37 Da
• Isoelectric Point: 8.5202

>AT239444 WP_072223385.1 NZ_CP094275:c43708-43475 [Klebsiella aerogenes]
MLINTPENIKILRKKIGLTQKECAEMFGMTARSWRRKEEPQTTASNTTLSPIEFNYLLLLAGEHPDYVLCKREALHP

Nucleotide

• Length: 234 bp

>AT239444 NZ_LR890719:827063-827329 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A223LMB9

Antitoxin

• AlphaFold DB: A0A223LLF6