Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 41752..42334 Replicon plasmid pNY1688-3
Accession NZ_CP094275
Organism Klebsiella aerogenes strain NY1688

Toxin (Protein)


Gene name PumA Uniprot ID E8PSG2
Locus tag MQE04_RS27510 Protein ID WP_013583078.1
Coordinates 42038..42334 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A223LLE5
Locus tag MQE04_RS27505 Protein ID WP_058998984.1
Coordinates 41752..42036 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MQE04_RS27470 (MQE04_27470) 37264..37557 - 294 WP_058998996.1 TrbC/VirB2 family protein -
MQE04_RS27475 (MQE04_27475) 37575..38204 - 630 WP_058998994.1 lytic transglycosylase domain-containing protein -
MQE04_RS27480 (MQE04_27480) 38211..38408 - 198 WP_058998992.1 hypothetical protein -
MQE04_RS27485 (MQE04_27485) 38398..38892 - 495 WP_058998990.1 transcription termination/antitermination NusG family protein -
MQE04_RS27490 (MQE04_27490) 39284..39691 + 408 WP_072223386.1 helix-turn-helix transcriptional regulator -
MQE04_RS27495 (MQE04_27495) 39688..40857 - 1170 WP_058998988.1 MobP1 family relaxase -
MQE04_RS27500 (MQE04_27500) 40861..41406 - 546 WP_058998986.1 hypothetical protein -
MQE04_RS27505 (MQE04_27505) 41752..42036 - 285 WP_058998984.1 putative addiction module antidote protein Antitoxin
MQE04_RS27510 (MQE04_27510) 42038..42334 - 297 WP_013583078.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MQE04_RS27515 (MQE04_27515) 42673..42810 - 138 WP_161400388.1 hypothetical protein -
MQE04_RS27520 (MQE04_27520) 42857..43069 - 213 WP_058998981.1 hypothetical protein -
MQE04_RS27525 (MQE04_27525) 43475..43708 - 234 WP_072223385.1 hypothetical protein -
MQE04_RS27530 (MQE04_27530) 43698..43955 - 258 WP_058998978.1 hypothetical protein -
MQE04_RS27535 (MQE04_27535) 43952..44494 - 543 WP_058998977.1 hypothetical protein -
MQE04_RS27540 (MQE04_27540) 44494..44700 - 207 WP_058998975.1 hypothetical protein -
MQE04_RS27545 (MQE04_27545) 44712..44927 - 216 WP_058998973.1 hypothetical protein -
MQE04_RS27550 (MQE04_27550) 44969..45490 - 522 WP_058998971.1 J domain-containing protein -
MQE04_RS27555 (MQE04_27555) 45603..45824 + 222 WP_058998969.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 - 1..46193 46193


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(46-89)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11290.10 Da        Isoelectric Point: 10.3166

>T239443 WP_013583078.1 NZ_CP094275:c42334-42038 [Klebsiella aerogenes]
MIEIKRTPEVEKWLRSLKDRTAKAKILMRLERMEDGNFGDVEPIGDGLSELRIHQGKGYRVYFGNKNNQIILLLCGGDKS
TQQADIRKAKQLAKQWGF

Download         Length: 297 bp

>T239443 NZ_LR890719:322711-323079 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10557.97 Da        Isoelectric Point: 4.7254

>AT239443 WP_058998984.1 NZ_CP094275:c42036-41752 [Klebsiella aerogenes]
MKEELKKFDVVEFLEDEEDIQEYLNAAIEEDDTKYLFKALGNIARARNISQLSKEVGMSREGIYKALSGEGNPSFQTVSK
ITKALGLKLHFTGA

Download         Length: 285 bp

>AT239443 NZ_LR890719:322493-322714 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A223LLP6

Antitoxin

Source ID Structure
AlphaFold DB A0A223LLE5

References