Detailed information of TA system TA239442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 103766..104421
• Replicon: plasmid pNY1688-2
• Accession: NZ_CP094274
• Organism: Klebsiella aerogenes strain NY1688

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: MQE04_RS27195
• Protein ID: WP_242925822.1
• Coordinates: 103766..104194 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: MQE04_RS27200
• Protein ID: WP_001261282.1
• Coordinates: 104191..104421 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQE04_RS27175 (MQE04_27175)	99869..100141	-	273	Protein_122	transposase	-
MQE04_RS27185 (MQE04_27185)	101123..102145	-	1023	WP_000361404.1	helicase UvrD	-
MQE04_RS27190 (MQE04_27190)	102130..103692	-	1563	WP_004206609.1	AAA family ATPase	-
MQE04_RS27195 (MQE04_27195)	103766..104194	-	429	WP_242925822.1	type II toxin-antitoxin system VapC family toxin	Toxin
MQE04_RS27200 (MQE04_27200)	104191..104421	-	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MQE04_RS27205 (MQE04_27205)	104378..104839	+	462	WP_014343465.1	hypothetical protein	-
MQE04_RS27210 (MQE04_27210)	105000..105944	+	945	WP_011977810.1	hypothetical protein	-
MQE04_RS27215 (MQE04_27215)	105981..106373	+	393	WP_011977811.1	hypothetical protein	-
MQE04_RS27220 (MQE04_27220)	106431..106952	+	522	WP_013214008.1	hypothetical protein	-
MQE04_RS27225 (MQE04_27225)	106998..107201	+	204	WP_011977813.1	hypothetical protein	-
MQE04_RS27230 (MQE04_27230)	107231..108235	+	1005	WP_011977814.1	hypothetical protein	-
MQE04_RS27235 (MQE04_27235)	108419..109198	+	780	WP_013214009.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..112047	112047

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-133)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15331.85 Da
• Isoelectric Point: 7.7526

>T239442 WP_242925822.1 NZ_CP094274:c104194-103766 [Klebsiella aerogenes]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWVRA
AVDATSGVVGSLLVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 429 bp

>T239442 NZ_LR890715:27274-27435 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACACCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT239442 WP_001261282.1 NZ_CP094274:c104421-104191 [Klebsiella aerogenes]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT239442 NZ_LR890715:c27221-27017 [Escherichia coli]
AGACATACAGGGGGCCTCGTGGGTTAATGAAAAATTAACTACGGGGCTTTCTGCCTTCTGCCACACAACACGGTAACAAA
CCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCGCACTGACCACACTCAC
TTTCCCTGAAAATAATCCGGTCGTTCAGCCAGTTCACGGGCAATC

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3