Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 42942..43467 Replicon plasmid pNY1688-1
Accession NZ_CP094273
Organism Klebsiella aerogenes strain NY1688

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag MQE04_RS26150 Protein ID WP_001159871.1
Coordinates 43162..43467 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag MQE04_RS26145 Protein ID WP_000813634.1
Coordinates 42942..43160 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MQE04_RS26110 (38148) 38148..38564 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
MQE04_RS26115 (38561) 38561..38791 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin -
MQE04_RS26120 (38748) 38748..38987 + 240 Protein_49 hypothetical protein -
MQE04_RS26125 (39136) 39136..40137 + 1002 WP_000785695.1 DUF4238 domain-containing protein -
MQE04_RS26130 (40311) 40311..40655 - 345 WP_000792769.1 hypothetical protein -
MQE04_RS26135 (41153) 41153..41407 - 255 WP_000678528.1 hypothetical protein -
MQE04_RS26140 (41457) 41457..42362 - 906 WP_224147498.1 hypothetical protein -
MQE04_RS26145 (42942) 42942..43160 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MQE04_RS26150 (43162) 43162..43467 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
MQE04_RS26155 (43468) 43468..44277 + 810 WP_000016963.1 site-specific integrase -
MQE04_RS26160 (44449) 44449..44643 + 195 Protein_57 colicin M immunity protein -
MQE04_RS26165 (44699) 44699..45396 + 698 WP_223200906.1 IS1-like element IS1A family transposase -
MQE04_RS26170 (45412) 45412..46002 - 591 Protein_59 colicin-like bacteriocin tRNase domain-containing protein -
MQE04_RS26175 (46182) 46182..46608 + 427 Protein_60 tyrosine-type recombinase/integrase -
MQE04_RS26180 (46800) 46800..47075 - 276 WP_000239529.1 hypothetical protein -
MQE04_RS26185 (47069) 47069..47713 - 645 WP_000633913.1 division plane positioning ATPase MipZ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / dfrA25 / qacE / sul1 / mph(A) / blaKPC-2 - 1..106628 106628
- flank IS/Tn - - 44893..45396 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T239438 WP_001159871.1 NZ_CP094273:43162-43467 [Klebsiella aerogenes]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T239438 NZ_LR890714:c4633884-4633534 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT239438 WP_000813634.1 NZ_CP094273:42942-43160 [Klebsiella aerogenes]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT239438 NZ_LR890714:c4634129-4633878 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References