Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 42942..43467 | Replicon | plasmid pNY1688-1 |
| Accession | NZ_CP094273 | ||
| Organism | Klebsiella aerogenes strain NY1688 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | S1PFV8 |
| Locus tag | MQE04_RS26150 | Protein ID | WP_001159871.1 |
| Coordinates | 43162..43467 (+) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | S1PPD8 |
| Locus tag | MQE04_RS26145 | Protein ID | WP_000813634.1 |
| Coordinates | 42942..43160 (+) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MQE04_RS26110 (38148) | 38148..38564 | - | 417 | WP_001044770.1 | type II toxin-antitoxin system VapC family toxin | - |
| MQE04_RS26115 (38561) | 38561..38791 | - | 231 | WP_001261282.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| MQE04_RS26120 (38748) | 38748..38987 | + | 240 | Protein_49 | hypothetical protein | - |
| MQE04_RS26125 (39136) | 39136..40137 | + | 1002 | WP_000785695.1 | DUF4238 domain-containing protein | - |
| MQE04_RS26130 (40311) | 40311..40655 | - | 345 | WP_000792769.1 | hypothetical protein | - |
| MQE04_RS26135 (41153) | 41153..41407 | - | 255 | WP_000678528.1 | hypothetical protein | - |
| MQE04_RS26140 (41457) | 41457..42362 | - | 906 | WP_224147498.1 | hypothetical protein | - |
| MQE04_RS26145 (42942) | 42942..43160 | + | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| MQE04_RS26150 (43162) | 43162..43467 | + | 306 | WP_001159871.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| MQE04_RS26155 (43468) | 43468..44277 | + | 810 | WP_000016963.1 | site-specific integrase | - |
| MQE04_RS26160 (44449) | 44449..44643 | + | 195 | Protein_57 | colicin M immunity protein | - |
| MQE04_RS26165 (44699) | 44699..45396 | + | 698 | WP_223200906.1 | IS1-like element IS1A family transposase | - |
| MQE04_RS26170 (45412) | 45412..46002 | - | 591 | Protein_59 | colicin-like bacteriocin tRNase domain-containing protein | - |
| MQE04_RS26175 (46182) | 46182..46608 | + | 427 | Protein_60 | tyrosine-type recombinase/integrase | - |
| MQE04_RS26180 (46800) | 46800..47075 | - | 276 | WP_000239529.1 | hypothetical protein | - |
| MQE04_RS26185 (47069) | 47069..47713 | - | 645 | WP_000633913.1 | division plane positioning ATPase MipZ | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaTEM-1B / dfrA25 / qacE / sul1 / mph(A) / blaKPC-2 | - | 1..106628 | 106628 | |
| - | flank | IS/Tn | - | - | 44893..45396 | 503 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11692.49 Da Isoelectric Point: 6.4674
>T239438 WP_001159871.1 NZ_CP094273:43162-43467 [Klebsiella aerogenes]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T239438 NZ_LR890714:c4633884-4633534 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8372.34 Da Isoelectric Point: 4.7405
>AT239438 WP_000813634.1 NZ_CP094273:42942-43160 [Klebsiella aerogenes]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT239438 NZ_LR890714:c4634129-4633878 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CCE8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CQY2 |