Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 38148..38791 Replicon plasmid pNY1688-1
Accession NZ_CP094273
Organism Klebsiella aerogenes strain NY1688

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag MQE04_RS26110 Protein ID WP_001044770.1
Coordinates 38148..38564 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag MQE04_RS26115 Protein ID WP_001261282.1
Coordinates 38561..38791 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MQE04_RS26090 (34251) 34251..34523 - 273 Protein_43 transposase -
MQE04_RS26100 (35505) 35505..36527 - 1023 WP_000361404.1 helicase UvrD -
MQE04_RS26105 (36512) 36512..38074 - 1563 WP_004206609.1 AAA family ATPase -
MQE04_RS26110 (38148) 38148..38564 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
MQE04_RS26115 (38561) 38561..38791 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MQE04_RS26120 (38748) 38748..38987 + 240 Protein_49 hypothetical protein -
MQE04_RS26125 (39136) 39136..40137 + 1002 WP_000785695.1 DUF4238 domain-containing protein -
MQE04_RS26130 (40311) 40311..40655 - 345 WP_000792769.1 hypothetical protein -
MQE04_RS26135 (41153) 41153..41407 - 255 WP_000678528.1 hypothetical protein -
MQE04_RS26140 (41457) 41457..42362 - 906 WP_224147498.1 hypothetical protein -
MQE04_RS26145 (42942) 42942..43160 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
MQE04_RS26150 (43162) 43162..43467 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / dfrA25 / qacE / sul1 / mph(A) / blaKPC-2 - 1..106628 106628


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T239437 WP_001044770.1 NZ_CP094273:c38564-38148 [Klebsiella aerogenes]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T239437 NZ_LR890714:c4622162-4621872 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT239437 WP_001261282.1 NZ_CP094273:c38791-38561 [Klebsiella aerogenes]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT239437 NZ_LR890714:c4622394-4622152 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References